中医药学报2017，Vol.45Issue(4)：70-74,5.
不同艾灸预处理时间对心肌缺血再灌注损伤大鼠心肌细胞中HIF-1α、Bcl-2及Caspase-3表达的影响
Effect of Different Length of Moxibustion Preconditioning on the Expression of HIF-1α, Bcl-2 and Caspase-3 in Myocardial Cells of MIRI Rats
摘要
Abstract
Objective: To discuss tHe protective mecHanism of moxibustion preconditioning for myocardiaL iscHemia reperfusion injury(MIRI), by observing tHe expression cHanges of HIF-1α, BcL-2, and Caspase-3 protein and mRNA in myocardiaL ceLLs of MIRI rats by different LengtH of moxibustion preconditioning.MetHods: 40 rats were randomLy divided into tHe normaL group, tHe modeL group, tHe moxibustion preconditioning group A(preconditioning for 5 days), and moxibustion preconditioning group B(preconditioning for 10 days).Moxibustion preconditioning was appLied on point of PC6 for 5 or 10 days respectiveLy before MIRI modeL being estabLisHed by coronary artery Ligation.THe expressions of HIF-1α, and Caspase-3 protein and mRNA in myocardiaL ceLLs were detected witH RT-PCR tecHnique and Western bLot metHod.ResuLts: THe protein and mRNA expressions of HIF-1α, and Caspase-3 in tHe modeL group was HigHer tHan tHe normaL LeveL(P<0.01);tHe protein and mRNA expressions of BcL-2 in tHe modeL group was Lower tHan tHe normaL LeveL (P<0.01).Compared to tHe modeL group, tHe expressions of HIF-1α and Caspase-3 in tHe moxibustion preconditioning groups decreased significantLy(P<0.05, P<0.01), tHe expressions of BcL-2 increased significantLy(P<0.01), of wHicH moxibustion preconditioning group B was superior to group A(P<0.05, P<0.01).ConcLusion: Moxibustion preconditioning can decrease tHe expression of HIF-1α and Caspase-3 and increase tHe expression of BcL-2 in myocardiaL ceLLs to improve myocardiaL iscHemia and inHibit myocardiaL apoptosis.THe protective effect of moxibustion preconditioning for 10 days was better tHan tHat for 5 days.关键词
艾灸预处理/心肌缺血再灌注损伤/低氧诱导因子-1α/B细胞淋巴瘤/白血病-2/天冬氨酸特异性半胱氨酸蛋白酶-3Key words
Moxibustion preconditioning/Myocardial ischemia reperfusion injury/HIF-1α/Bcl-2/Caspase-3分类
医药卫生引用本文复制引用
王超,刘薇薇,黄洁,刘密,谭成富,常小荣,严洁..不同艾灸预处理时间对心肌缺血再灌注损伤大鼠心肌细胞中HIF-1α、Bcl-2及Caspase-3表达的影响[J].中医药学报,2017,45(4):70-74,5.基金项目
2015年针灸推拿学湖南省"十二五"重点学科开放基金资助项目(06) （06）
国家自然科学基金项目(81574080) （81574080）
湖南省中管局课题(201521) （201521）
湖南省高校创新平台开放基金项目(15k093) （15k093）
