中医药信息2017，Vol.34Issue(4)：26-28,3.
金银花UPLC指纹图谱及其次生代谢物化学成分比较研究
Study on UPLC Fingerprint of Flos Lonicerae and the Chemical Composition of the Secondary Metabolites
摘要
Abstract
Objective:To establish the fingerprint spectrum of Flos lonicerae by HPLC technique.Methods:With chlorogenic acid as reference, the fingerprint was established on Acquity UPLCTM BEH C18 Column (2.1 mm×50 mm, 1.7 μm).Acetonitrile-0.1% formic acid was used as gradient mobile phase at the flow rate of 0.3 mL/min.The column temperature was 40℃.The chromatograms of Flos lonicerae and its secondary metabolites were analyzed, and its quality was evaluated by Chinese Medicine Chromatographic Fingerprint Evaluation System (2004 A).Results: Common mode of the fingerprints was established from 10 batches of Flos lonicerae.There were total 10 common spectrum peaks taking chlonogenic acid as reference, which were identified with better separation.Conclusion: The method is time-saving, efficient and can be used for the quality control of Flos lonicerae.关键词
金银花/次生代谢物/绿原酸/UPLC/指纹图谱Key words
Flos lonicerae(Jinyin Hua)/Secondary metabolites/Chlorogenic acid/UPLC/Fingerprint分类
医药卫生引用本文复制引用
石朗,王丹丹,杨丽军,李杉杉,刘健,陈大忠..金银花UPLC指纹图谱及其次生代谢物化学成分比较研究[J].中医药信息,2017,34(4):26-28,3.基金项目
黑龙江省中医药科研项目(No.ZHY16-099) （No.ZHY16-099）
黑龙江省应用技术研究与开发计划项目(No.GC13C109) （No.GC13C109）
