中医药信息2017，Vol.34Issue(5)：28-33,6.
花旗泽仁对胰岛素抵抗大鼠肝脏CaSR mRNA、蛋白表达及AKT活性的影响
Influence of Huaqizeren on the Expressions of Protein CaSR, CaSR mRNA, and Protein AKT in the Liver Tissue of T2DM Rats with IR
摘要
Abstract
Objective:To observe the influence of Huaqizeren(HQZR) on the expressions of protein CaSR(calcium-sensing receptor),CaSR mRNA,Protein AKT (Kinase B) of the liver tissues in rats with insulin resistance(IR),in order to explore the mechanism of HQZR improving T2DM (Type 2 Diabetes Mellitus) with IR.Methods:The IR model was established with compound fat emulsion diet for four weeks and small-dose STZ injection in male Wistar rats.The rats were randomly divided into the normal control group,the model group,the HQZR group,and the positive control group.FBG(Fasting blood glucose) and FINS(fasting insulin) were tested and ISI (insulin sensitivity index) was calculated.The expressions of CaSR mRNA,protein CaSR and phosphorylated AKT(Ser473 and Thr308) were detected with qRT-PCR and Western blot techniques.Results:In terms of the levels of FBG,FINS,and ISI,FBG and FIN were significantly increased with decreased ISI in the model group,compared to the normal control group after medication;FBG and FINS were significantly decreased with increased ISI in both the HQZR group and the positive control group compared to the normal group.In terms of the expression of CaSR mRNA,it was significantly decreased in the model group compared to the normal control group;it was significantly increased after the treatment in both the HQZR group and the positive control group.In terms of protein expressions of CaSR and phosphorylated AKT,they were decreased after modeling,whereas they were significantly increased after medication both in the HQZR group and in the positive control group.Conclusion:HQZR may improve IR by regulating CaSR mRNA,protein CaSR and AKT.关键词
2型糖尿病/胰岛素抵抗/钙敏感受体/AKT/花旗泽仁Key words
Type 2 diabetes mellitus(T2DM)/Insulin resistance(IR)/Calcium-sensing receptor (CaSR)/AKT/Huaqizeren(HQZR)分类
医药卫生引用本文复制引用
刘笑男,李璐,赵聪,郑义,阚玉娜,刘萍,葛鹏玲..花旗泽仁对胰岛素抵抗大鼠肝脏CaSR mRNA、蛋白表达及AKT活性的影响[J].中医药信息,2017,34(5):28-33,6.基金项目
国家科技重大专项(No.2012ZX09103201-018) （No.2012ZX09103201-018）
国家自然科学基金项目(No.81273650) （No.81273650）
黑龙江省自然科学基金项目(No.LC2011C03) （No.LC2011C03）
黑龙江中医药大学"优秀创新人才支持计划"(No.2012RCD19) （No.2012RCD19）
黑龙江省博士后科研启动基金项目(No.LBH-Q15136) （No.LBH-Q15136）
哈尔滨市科技创新人才研究专项资金项目(No.2016RAXXJ100) （No.2016RAXXJ100）
黑龙江中医药大学科研基金项目(No.201515) （No.201515）
