安徽农业科学2017，Vol.45Issue(25)：145-148,150,5.
安徽凤阳玉米穗腐病病原菌的分离鉴定
Isolation and Identification of the Pathogen of Maize Ear Rot in Fengyang,Anhui Province
摘要
Abstract
[Objective] The aim was to know the pathogens causing maize ear rot in Fengyang.[Method] The maize ear rot samples were collect-ed from Fengyang County.The tissue isolation method was adopted to isolate the maize disease samples,and then purified them.The pathogens were identified based on results of morphological characteristics and molecular biology.[ Result] Fifty-six isolates were identified as members of Fusarium gra minearum,Fusarium oxysporum,Fusarium verticillioide,Rhizomucor variabilis and Penicillium spp.,and the isolation frequency were 44.64%,21.42%,14.29%,14.29% and 5.36%,respectively.In this experiment,Fusarium gra minearum was the predo minant species in maize ear rot.The Fusarium gra minearum Fg21 could grow under 5-30 ℃,with optimal condition at 25 ℃,pH 7.0;the Fusarium oxysporum Fa09 could grow unde 10-35℃,with optimal condition at 28℃,pH 7.0;the Fusarium verticillioide Fv03 could grow unde 10-35℃,with opti-mal condition at 25℃,pH 10.0.Vegetative growth of Fg21,Fa09 and Fv03 were found to be significantly influenced by nitrogen source compared with carbon source.[Conclusion] The results provide theoretical basis for comprehensive prevention and control of maize ear rot.关键词
玉米穗腐病/病原鉴定/生物学特性Key words
Maize ear rot/Isolation of pathogenic bacteria/Biological characteristics分类
农业科技引用本文复制引用
许大凤,张海珊,李廷春,齐耀程,姜超强,周本国..安徽凤阳玉米穗腐病病原菌的分离鉴定[J].安徽农业科学,2017,45(25):145-148,150,5.基金项目
安徽省农业科学院学科建设项目(15A0932). （15A0932）
