重庆医学2017，Vol.46Issue(25)：3477-3479,3483,4.DOI:10.3969/j.issn.1671-8348.2017.25.007
3种方法治疗肠道菌群失调合并过敏性哮喘大鼠对肠道菌群的影响
Influence of three different therapeutic methods on intestinal flora in treating rat intestinal dysbacteriosis complicating allergic asthma
摘要
Abstract
Objective To provide a theoretical basis for the pulmonary enteric therapy in treating intestinal dysbacteriosis complicating allergic asthma according to the traditional theory of "Lung and Large intestine are interiorly-exteriorly".Methods The experiment included the blank group,model group,positive control group and three kinds of traditional Chinese medicine compound administration group;the intestinal dysbacteriosis in SD rats was duplicated by adopting cefoperazone and candida albicans gavage.On this basis ovalbumin injection sensitization and nebulization were applied to induce asthma.Meanwhile Jia-Wei-ShengJiang Powder(SJS),Guizhi-Jia-Houpu-Xingzi Decoction(GHX) and Zeng-Ye-Cheng-Qi Decoction(ZYCQ) were orally administered for treatment of lung-intestine combine therapy,lung therapy and intestine therapy.The respiratory function was analyzed by using the Buxco whole body plethysmography.The variety of intestinal flora were analyzed by using 16SrDNA.Results Analyzing the specieces composition of intestinal flora in each group by the OUT value found that the recovery of Lactobacillus in each group was close to that in the blank group and better than that in the positive group,meanwhile,3 treatment groups all could promote the recovery of Proteobacteria and bacteroidetes,but the lung-intestine combine therapy had optimal effect,the Alpha diversity analysis found that the intestinal microflora diversities from high to low were in turn the intestinal therapy group,lung therapy group,lungintestine combined therapy group and model group;the respiratory frequency and airway hyperreactivity in the model group was 260.56(1.28),which in the lung-intestine combined therapy group,lung therapy and intestine therapy after treatment were 141.79 (0.93),137.25 (1.30) and 203.16 (0.84) respectively,the rat respiratory frequency in each treatment group was lower than that in the model group,while the airway hyperreactivity only in the lung-intestine combined therapy group and intestinal therapy group was lower than that in the model group,moreover showing statistical significance.Conclusion The lung-intestine combine therapy method for treating rat intestinal dysbacteriosis complicating allergic asthma is superior to simple lung therapy method or intestine therapy method.关键词
哮喘/肠道菌群/中医治疗学Key words
asthma/intestinal microflora/therapeutics分类
医药卫生引用本文复制引用
付雯,刘兴隆,张珑琼,高永翔..3种方法治疗肠道菌群失调合并过敏性哮喘大鼠对肠道菌群的影响[J].重庆医学,2017,46(25):3477-3479,3483,4.基金项目
国家自然科学基金资助项目(81303085) （81303085）
成都中医药大学科技发展基金资助项目(ZRYY1620). （ZRYY1620）
