重庆医学2017，Vol.46Issue(25)：3480-3483,4.DOI:10.3969/j.issn.1671-8348.2017.25.008
晚期氧化蛋白产物调节人肾小管上皮细胞自噬的研究
Advanced oxidation protein products modulate autophagy of renal tubule epithelial cells
摘要
Abstract
Objective To investigate the effect of advanced oxidation protein products (AOPPs) on human renal tubular epithelial cells(HK-2) autophagy.Methods HK-2 cells were stimulated with AOPPs.RT-qPCR and Western blot were used to determine the expression of autophagy related protein LC3-Ⅱ/LC3-Ⅰ,Beclin1 and p62;Western blot was utilized to examine the activation of p38 MAPK pathway.Then p38 MAPK inhibitor (SB203580) was added and co-processed with AOPPs.The change of autophagy was observed Also,autophagy inducer rapamycin was added and co-processed with AOPPs.RT-qPCR and Western blot were used to detect the expression of cell cycle inhibitory protein p27.The cell total protein level was detected by the bicinchoninic acid (BCA) method.The hypertrophy change was observed.Results AOPPs down-regulated the expression of LC3-Ⅱ/LC3-Ⅰ and Beclin1,up-regulated expression of p62 and activated p38 MAPK pathway;in comparison with the AOPPs alone treatment group,the expression of LC3-Ⅱ/LC3-Ⅰ and Beclin in the SB203580 co-processing group was increased,while p62 was decreased;the p27 expression and cells total protein in the sirolimus co-processing group were down-regulated.Conclusion AOPPs inhibits the autophagy of HK-2 cells by activating p38 MAPK pathway and the autophagy inhibition participates in HK-2 cell hypertrophy.关键词
自噬/p38 MAPK信号通路/晚期氧化蛋白产物Key words
autophagy/p38 MAPK signaling pathway/advanced oxidation protein products分类
医药卫生引用本文复制引用
章俊,向晓红,梁秀洁,束双双,姜婷婷,郭婷婷,汤珣..晚期氧化蛋白产物调节人肾小管上皮细胞自噬的研究[J].重庆医学,2017,46(25):3480-3483,4.基金项目
国家自然科学基金资助项目(81202842). （81202842）
