动物营养学报2017，Vol.29Issue(9)：3211-3219,9.DOI:10.3969/j.issn.1006-267x.2017.09.024
大菱鲆幼鱼对玉米蛋白粉中营养物质的表观消化率及添加胆汁酸和酶制剂对其产生的影响
Nutrient Apparent Digestibility Coefficients of Corn Gluten Meal for Juvenile Turbot (Scophthalmus maximus L.) and Effects of Adding Bile Acid and Enzyme Preparation on Them
摘要
Abstract
This experiment was conducted to determine the apparent digestibility coefficients (ADCs) of dry matter (DM),crude protein (CP),amino acid (AA) and gross energy (GE) of corn gluten meal,and the effects of adding bile acid (purity≥30%) and enzyme preparation [compound enzyme which was composed of neutral protease (1×105 U/g) and papain (4×105 U/g),and the activity ratio of them was 2∶1] on ADCs of nutrients in corn gluten meal.A basal diet was prepared firstly,and then the test diets were prepared which contained 70% basal diet and 30% test feed ingredients [untreated corn gluten meal (CGM),corn gluten meal supplemented with bile acid (CGMB),and corn gluten meal supplemented with enzyme preparation (CGME)].All diets were added 0.1% yttrium trioxide as an exogenous indicator.Juvenile turbot with the average body weight of (13.00±0.01) g were randomly divided into 4 groups with 3 replicates in each group and 40 fish in each replicate.The fish in different group were fed with the corresponding diet,and fecal samples were collected with the method of squeezing the hindgut after 2 weeks.The feeding period was 10 weeks.The results showed that the ADCs of DM,CP,total AA and GE of three protein sources were 25.99% to 43.34%,48.62% to 60.72%,48.41% to 67.67%,and 35.07% to 52.34%,respectively.The ADC of total AA of each protein source presented the similar tendency with the ADC of CP.CGMB showed the highest ADCs of all nutrients for juvenile turbot,which were significantly higher than those of CGM and CGME (P<0.05),and the ADCs of DM,CP,total AA and GE of CGMB were increased by 17.35%,12.10%,19.26% and 17.27%,respectively,compared with CGM;the ADCs of all nutrients of CGME for juvenile turbot were also improved,the ADCs of DM,CP,total AA and GE of CGMB were increased by 9.53%,4.37%,4.29% and 12.25%,respectively,compared with CGM,and the effects were significant (P<0.05).The results indicate that adding bile acid and enzyme preparation all contribute to the improvement of ADCs of nutrients of corn gluten meal for juvenile turbot.关键词
大菱鲆幼鱼/玉米蛋白粉/胆汁酸/酶制剂/表观消化率Key words
juvenile turbot/corn gluten meal/bile acid/enzyme preparation/apparent digestibility coefficient分类
农业科技引用本文复制引用
田莹,何艮,周慧慧,王旋,麦康森..大菱鲆幼鱼对玉米蛋白粉中营养物质的表观消化率及添加胆汁酸和酶制剂对其产生的影响[J].动物营养学报,2017,29(9):3211-3219,9.基金项目
公益性行业专项(201303053)--替代鱼用饲料中鱼粉的新蛋白源开发利用技术 （201303053）
