辐射防护2017，Vol.37Issue(4)：309-316,8.
黄芪甲苷对肝细胞放射损伤预防作用的机制研究
Mechanism of radioprotection effects of Astragaloside Ⅳ on the liver cell
摘要
Abstract
The radioprotection effects and molecular mechanism of Astragaloside Ⅳ (ASIV) on the liver cell L -02 was studied.The experiments were conducted with four groups:the control group,the ASIV group,the irradiation group and the ASIV + irradiation group.The rate of apoptosis and cell cycle were detected by flow cytometry and Rhodamine-123 fluorescent probe was used to detect potential alternation of the mitochondrial membrane in L-02 cell.γH2AX foci were detected by immunofluorescence confocal microscopy while the expressions of Nrf2,HO-2,NQO1 proteins were observed by western blot method.The results showed that the apoptosis rate and the arrested cell cycle G2 decreased with increasing concentrations of Astragaloside Ⅳ after irradiation and the membrane potential increased with the rise of Astragaloside Ⅳ concentration as observed by spectrophotofluorimetry after Rh123 dyeing.The confocal microscopy results showed that the number of γH2AX focus in Astragaloside + irradiation group was significantly less than in the irradiation group.Western Blot results indicated that the expression of Nrf2,HO 1-and NQO1 increased in the irradiated cells,while the opposite trend showed in Astragaloside + irradiation group with increasing drug concentration.关键词
黄芪甲苷/凋亡/细胞周期/γH2AX/Nrf2Key words
Astragaloside Ⅳ/apoptosis/cell cycle/γH2AX/Nrf2分类
生物科学引用本文复制引用
胡雅梦,龙颖,陈科良,刘奔波,何淑雅,黄波..黄芪甲苷对肝细胞放射损伤预防作用的机制研究[J].辐射防护,2017,37(4):309-316,8.基金项目
国家自然科学基金项目(No.81272994) （No.81272994）
湖南省自然科学基金项目(2016JJ2115) （2016JJ2115）
湖南省卫生计生委科研计划课题项目(C2015-17) （C2015-17）
南华大学博士启动基金(2015XQD31) （2015XQD31）
放射生物学北京市重点实验室开放课题(BKLR2016-01). （BKLR2016-01）
