光学精密工程2017，Vol.25Issue(8)：2029-2037,9.DOI:10.3788/OPE.20172508.2029
利用光压差分技术筛选细胞的影响参数
Analysis on influence parameters of cell sortingusing optical pressure difference technology
摘要
Abstract
In this paper, the finite volume method was adopted to establish cross cell separation model, and a cell screening simulation method was proposed based on light pressure difference for analysis of influence factors of cell screening in microfluidics.Based on laminar flow, fluid flow, particle tracking and wave optics theories, a cross type optical particle separation model was established by finite element analysis.Using light pressure difference technology, various factors affecting the separation of cells were studied, including particle diameter, laser power, temperature and fiber diameter.Furthermore, deflection distances of the particles in the fluid due to the optical radiation pressure were determined experimentally.The results show that in microfluidics, laser power, cell diameter and temperature (20 ℃) generally are proportional to deflection distance separately.When fiber diameter and cell diameter are in the same size, optical radiation pressure reaches the maximum.When laser acts on particles with diameter of 3 μm, 8 μm and 20 μm through fibers with diameters of 7 μm or 8 μm, radiation pressure reaches its maximum, thus choosing single mode fiber with diameter of 8 μm as an important optical device in the experiments.The above conclusion provides an reference for further study of precision of numerical simulation on cell screening factors.关键词
细胞分离/光纤/光辐射压力/光压差分/数值仿真Key words
cell sorting/optical fiber/optical radiation pressure/optical pressure difference/numerical simulation分类
机械制造引用本文复制引用
程景萌,杨丽,周围,李新冉,张思祥..利用光压差分技术筛选细胞的影响参数[J].光学精密工程,2017,25(8):2029-2037,9.基金项目
河北省青年科学基金资助项目(No.B2014202260) （No.B2014202260）
