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空间环境诱导小麦变异及ISSR多态性研究

蒋云 张洁 郭元林 尹春蓉 宣朴

核农学报2017,Vol.31Issue(9):1665-1671,7.
核农学报2017,Vol.31Issue(9):1665-1671,7.DOI:10.11869/j.issn.100-8551.2017.09.1665

空间环境诱导小麦变异及ISSR多态性研究

Space Environment Induced Mutations and ISSR Polymorphism in Wheat

蒋云 1张洁 2郭元林 1尹春蓉 1宣朴1

作者信息

  • 1. 四川省农业科学院生物技术核技术研究所,四川 成都 610061
  • 2. 四川农业大学小麦研究所,四川 温江 611130
  • 折叠

摘要

Abstract

To evaluate the effect of space environment mutagenesis on agronomic traits and molecular-level variation in wheat,this experiment investigated the agronomic traits in the SP1 generation and polymorphism of inter simple sequence repeat (ISSR) markers in the SP4,SP5 generations of three wheat cultivars (lines) carried by the recoverable satellite Shijian-8.The results showed that:After space environment mutagenesis,the agronomic traits of SP1 generation were significantly different from those of the wild type,and different wheat genotypes showed differential sensitivity to the space environment mutagenesis.The average genetic similarity index for the mutant lines and the wild type ranged from 0.8226 to 0.8777.At the DNA level,multiple loci in the mutants differed from those in the wild type.The inconsistent results of the sensitivity to space environment of SP1 and Nei's genetic distance between the mutants and the wild type may be explained by the high frequency of advantageous mutations resulting from artificial selection.Overall,this study illustrated that the space environment mutagenesis can induce mutations on molecular level in wheat,and can provide several excellent breeding materials for space breeding of wheat in China.

关键词

空间环境诱变/小麦/农艺性状/ISSR标记

Key words

space environmental mutagenesis/wheat/agronomic traits/ISSR markers

引用本文复制引用

蒋云,张洁,郭元林,尹春蓉,宣朴..空间环境诱导小麦变异及ISSR多态性研究[J].核农学报,2017,31(9):1665-1671,7.

基金项目

国家重点研发计划“主要农作物诱变育种”项目(2016YFD0102101),四川省财政创新能力提升工程项目(2016ZYPZ-004),“十三五”四川省公益性育种研究专项项目(2016NYZ0030) (2016YFD0102101)

核农学报

OA北大核心CSCDCSTPCD

1000-8551

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