核农学报2017，Vol.31Issue(9)：1811-1817,7.DOI:10.11869/j.issn.100-8551.2017.09.1811
外源水杨酸缓解低温胁迫对玉米种子萌发和早期幼苗生长伤害的生理机制
Physiological Mechanisms of Exogenous Salicylic Acid-mediated Low Temperature Tolerance in Seed Germination and Early Seedling Growth of Maize
摘要
Abstract
In this study,the maize cultivar Xianyu 335 hybrid was used to investigate the physiological mechanism of SA on seed germination and early seedlings growth under low temperature condition.The maize seed soaking treatments were performed with 0.05 mmol· L-1 SA and deionized water (control,CK),and then we studied the effects of SA on maize seed germination,amylase activity,oxidative damage,protective enzyme activities and osmotic adjustment substances content under low temperature stress.The results showed that,compared with the control,seed germination rate,activity index,vigor index,fresh weight and dry weight of seedlings in SA-treated maize were significantly increased by 5.57％,7.20％,23.76％,15.49％ and 28.52％,respectively.In SA-treated maize seed embryo,the amylase activity was significantly increased by 28.6％ 、15.1％ 、9.6％ and 16.9％,respectively,at 2,6,8 and 10 d after seed germination.However,the levels of superoxide anion free radical (O2·-) and malondialdehyde (MDA) were significantly decreased.Further research suggested that SA-treated improved antioxidant enzyme activity,superoxide dismutase (SOD) activity was increased by 36.6％ 、25.9％ 、43.6％ and 46.3％,respectively,at 2,4,6 and 8 d after seed germination.Peroxidase (POD) activity was increased by 9.0％ at 8 d after seed germination.Catalase (CAT) was increased activity by 13.0％,5.7％ and 17.0％,respectively,at 4,6,8d after seed germination.Moreover,the contents of glutathione (GSH) and osmotic adjustment substances (including proline,soluble protein,and soluble sugar) were improved by SA treatment.Taken together,SA could alleviate low temperature stress-induced oxidative damage by improving the osmotic adjustment ability and accelerating starch conversion.This findings provide an insight into the mechanism how SA regulates seed germination and early seedling growth under low temperature stress.关键词
水杨酸(SA)/低温胁迫/玉米/种子萌发/氧化伤害Key words
salicylic acid (SA)/low-temperature stress/maize/seed germination/oxidative damage引用本文复制引用
杨小环,赵维峰,孙娜娜,孙亮亮,马金虎..外源水杨酸缓解低温胁迫对玉米种子萌发和早期幼苗生长伤害的生理机制[J].核农学报,2017,31(9):1811-1817,7.基金项目
国家自然科学基金项目(31200914),中国博士后科学基金(2012 M520600) （31200914）
