核农学报2017，Vol.31Issue(9)：1827-1835,9.DOI:10.11869/j.issn.100-8551.2017.09.1827
8个新疆野苹果优良无性系抗寒性比较
Comparison of Cold Hardiness of 8 Malus sieversii Clones
摘要
Abstract
To compare the difference of cold resistance of 8 Malus sieversii clones in different periods (January and March),and select the high-quality resources of Malus sieversii,breeding for cold resistance,the cold resistance of 8 Malus sieversii clones was measured and compared by electrical conductivity method (EL) and electrical impedance spectroscopy (EIS) in two periods.The germination experiment was carried out after low temperature treatment in March.Both methods can calculate the cold resistance of Malus sieversii,according to the calculated semi-lethal temperature and germination percentage after low temperature treatment.The results showed that the relative conductivity of 8 Malus sieversii clones were significantly different at-20℃ in the periods.The semi-lethal temperature calculated by re,r1 and the conductivity method were significantly correlated in the periods(0.744*,0.751*,0.822**,0.824**);The semi-lethal temperature in March (-33.97℃ ～-32.33℃) was significantly higher than that in January (-31.87℃ ～-26.33℃),and the difference among some clones reached the extremely significant level.The semi-lethal temperature of each clone had no significant correlation at 2 periods;The calculated semi-lethal temperature of clones M -3,M-6 and M-8 were lower which were-32.76℃,-33.97℃,-33.52℃ in January,and-30.47℃,-30.26℃,-31.87℃ in March.The germination rate of clones M-3,M-6 and M-8 were higher in the growth and recovery experiment in March which were 76.19％,75.71％ and 67.38％ under-30℃ and 16.67％,17.72％ and 18.89％ at-50℃.Clones M-3,M-6 and M-8 had better performance in 2 periods'cold resistance and germination experiment,which are the good resources for cold-resistant breeding.The results provide a scientific basis for further study of cold hardiness of Malus sieversii in different periods and select high-quality resources of cold-resistant breeding.关键词
新疆野苹果/无性系/抗寒性/半致死温度Key words
Malus sieversii/clone/cold hardiness/semilethal temperature引用本文复制引用
于立洋,李政,韩佩尧,张静,汪敏骅,田晓晓,张军..8个新疆野苹果优良无性系抗寒性比较[J].核农学报,2017,31(9):1827-1835,9.基金项目
木本植物品种DNA分子图谱研究(XPC-201505) （XPC-201505）
