老年医学与保健2017，Vol.23Issue(4)：286-289,4.
白细胞介素-1β促进人真皮成纤维细胞分泌促血管生成及炎症因子
IL-1β Promotes the Secretion of Angiogenic and Inflammatory Factors in Human Dermal Fibroblast
摘要
Abstract
Objective To explore the role of interleukin-1β (IL-1β) in inhibiting the growth of human dermal fibroblast (HDF) and in promoting HDF secretion of angiogenic and inflammatory factors.Methods CCK8 was applied in detecting the growth inhibiting rate of HDF treated with IL-1β of different concentrations while ELISA was applied in detecting the expression levels of vascular endothelial growth factor (VEGF),fibroblast growth factor (FGF) and IL-6 in HDF treated with 3 and 10ng/mL IL-1β for 24 hours.Results The growth inhibiting rate of HDF treated with 0.3ng/mL IL-1β,1 ng/mL IL-1β,3 ng/mL IL-1β and 10ng/mL IL-1β was (1.22±0.78) ％,(3.72±1.21) ％,(9.10±2.60) ％ and (14.42±3.36) ％ respectively;the expressions of VEGF and FGF in 3 and 10ng/ml IL-1[β group increased significantly and were higher than those in blank control group (VEGF P＜0.01,FGF P＜0.05) while there existed no statistical difference in the expressions between 3 and 10ng/ml IL-1β group;the expressions of IL-6 in 3 and 10ng/ml IL-1β group were higher than that in blank control group (P＜0.05,P＜0.001) while the expression of IL-6 in 10ng/ml IL-1β group was much higher than that in 3ng/ ml IL-1β group (P＜0.001).Conclusions IL-1β can promote HDF secretion of VEGF,FGF and inflammatory cytokine IL-6,which indicates that IL-1β may promote the tumorigenesis,invasion and metastasis..关键词
人真皮成纤维细胞/白介素-1β/成纤维细胞生长因子/血管内皮生长因子/白介素-6Key words
human dermal fibroblast (HDF)/interleukin-1β (IL-1β)/fibroblast growth factor (FGF)/vascular endothelial growth factor (VEGF)/IL-6引用本文复制引用
潘毛毛,吕婷,张洁尘,聂舒,缪飞,王宏伟..白细胞介素-1β促进人真皮成纤维细胞分泌促血管生成及炎症因子[J].老年医学与保健,2017,23(4):286-289,4.基金项目
国家自然科学基金(81572671) （81572671）
