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常温降解玉米秸秆复合菌系及其 微生物多样性研究

邓兵 贾军 艾士奇 晏磊 高亚梅 王彦杰 王伟东

可再生能源2017,Vol.35Issue(8):1127-1134,8.
可再生能源2017,Vol.35Issue(8):1127-1134,8.

常温降解玉米秸秆复合菌系及其 微生物多样性研究

The screening and diversity of a bacterial community capable of degrading corn straw at 30℃

邓兵 1贾军 1艾士奇 1晏磊 1高亚梅 1王彦杰 1王伟东1

作者信息

  • 1. 黑龙江八一农垦大学 生命科学技术学院,黑龙江 大庆 163319
  • 折叠

摘要

Abstract

In order to promote corn stalk degrading under normal temperature, a bacterial community with high efficiency lignocellulose degrading, sampling from corn soil, was screened through continuous restricted cultivation and the degradation rate of cellulose as the main indicator. The optimum temperature and pH for lignocellulose degradation was 30 ℃ and 8, respectively. Under the condition above, the degrading rate of filter and corn straw was 66.5% and 66.1%respectively. Among the lignocellulose degradation rate of corn straw, the degradation rate of cellulose, hemicelluloses and lignin was 17.0%, 85.1%, 34.4%, respectively. The results of microbial diversity by high -throughput sequencing showed that the bacterial community was mainly composed of Pseudomonas(33.9%), Bacteroides (11.8%), Acidovorax (6.9%), Alcaligenes (3.8%), Butyrivibrio (1%) at the genus level. At the same time, the consortium was mainly composed of Alcallgenes-faecalis (2%), Microvirgula-aerodenitrificans (1.5%) at the species level. Bacterial community can efficiently decompose hemicelluloses, cellulose and other ingredients under normal temperature, and there is the potential for agricultural waste recycling.

关键词

常温/复合菌系/玉米秸秆/木质纤维素/微生物多样性

Key words

normal temperature/bacterial community/corn stalk/lignocellulose/microbial diversity

分类

能源科技

引用本文复制引用

邓兵,贾军,艾士奇,晏磊,高亚梅,王彦杰,王伟东..常温降解玉米秸秆复合菌系及其 微生物多样性研究[J].可再生能源,2017,35(8):1127-1134,8.

基金项目

"十二五"国家科技支撑计划项目(2016YFD0800602,2015BAD21B04,2012BAD12B05-3) (2016YFD0800602,2015BAD21B04,2012BAD12B05-3)

黑龙江省高校科技创新团队项目(2012TD006) (2012TD006)

黑龙江农垦总局科技攻关项目(HNK125B-11-06A,HNK135-04-08). (HNK125B-11-06A,HNK135-04-08)

可再生能源

OA北大核心CSTPCD

1671-5292

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