中国组织工程研究2017，Vol.21Issue(25)：3949-3955,7.DOI:10.3969/j.issn.2095-4344.2017.25.003
脐带间充质干细胞对NB4白血病细胞基因组表达谱的影响
Genome-wide transcriptional profiling of NB4 leukemic cells affected by umbilical cord-derived mesenchymal stem cells
摘要
Abstract
BACKGROUND: Mesenchymal stem cells (MSCs) are an important component of the in vivo microenvironment and act on multiple biological behaviors of tumor cells. The potential clinical value of MSCs has become an issue of concern in recent years.OBJECTIVE: To investigate the gene expression profiles of acute promyelocytic leukemia (APL) cell line NB4 treated with umbilical cord-derived MSCs (UC-MSCs) using cDNA microarray.METHODS: In vitro co-culture system was constructed, and then cellular proliferation, apoptosis and differentiation status of NB4 cells treated with UC-MSCs were evaluated. Two cDNA probes were prepared through reverse transcription from mRNA of NB4 cells treated with or without UC-MSCs. The probes were labeled with fluorescence dyes individually, hybridized with cDNA microarray, and their fluorescent intensities were scanned. The genes were screened through the analysis of the difference in two gene expression profiles.RESULTS AND CONCLUSION: UC-MSCs promoted the proliferation and differentiation, while reduced the apoptosis of NB4 cells. The analysis of gene expression profiles indicated that after co-culture with UC-MSCs, 530 genes were up-regulated and 53 genes were down-regulated. Accordingly, specific gene function and pathway signaling related were also regulated to some extent. Overall, UC-MSCs influence can major biological behaviors of NB4 cells by changing expression of a large amount of genes, gene-related function and multiple intracellular signaling pathways.关键词
干细胞/脐带脐血干细胞/白血病/NB4细胞/脐带间充质干细胞/cDNA芯片/国家自然科学基金分类
医药卫生引用本文复制引用
范会芳,陈芳,马凤霞,池颖,卢士红,韩忠朝..脐带间充质干细胞对NB4白血病细胞基因组表达谱的影响[J].中国组织工程研究,2017,21(25):3949-3955,7.基金项目
国家自然科学基金(81500098和81330015) （81500098和81330015）
中国医学科学院医学与健康科技创新工程(2016-I2M-1-017) the National Natural Science Foundation of China, No. 81500098, 81330015 （2016-I2M-1-017）
the CAMS Initiative for Innovative Medicine, No. 2016-I2M-1-017 （）
