中国组织工程研究2017，Vol.21Issue(25)：3964-3970,7.DOI:10.3969/j.issn.2095-4344.2017.25.005
聚乙烯亚胺递送miR-218及miR-26a对骨髓间充质干细胞成骨分化的影响
Effects of miR-218 and miR-26a on osteogenic differentiation of bone marrow mesenchymal stem cells by polyethyleneimine delivery
摘要
Abstract
BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are widely used in the field of tissue engineering because of their multi-directional differentiation potential. Micro RNAs play an important role in promoting osteogenic differentiation of BMSCs.OBJECTIVE: To investigate the effect of miR-218 and miR-26a on the osteogenic differentiation of rat BMSCs, and to provide reference for the study on osteogenic differentiation of BMSCs and the clinical application. METHODS: The bone marrow of the femur of Wistar rats was extracted and the BMSCs were isolated and cultured to the 3rd generation. MiR-218, miR-26a and polyethyleneimine (PEI) were mixed in a specific ratio to form a miRNA/PEI complex. Meanwhile, a negative control group was established.RESULTS AND CONCLUSION: (1) Rat BMSCs grew well. (2) The optimal concentration of miRNA mimics was 50 nmol/L by MTT method. (3) The expression of alkaline phosphatase and collagen type I mRNA was significantly increased (P < 0.05). (4) Alkaline phosphatase staining showed that compared with the blank control group and the negative control group, the cytoplasm showed obvious coloring. (5) There were a lot of mineralized nodules shown by alizarin red staining. Therefore, miR-218/PEI complex, miR-26a/PEI complex and miR-218+miR-26a/PEI co-transfection complex could promote the osteogenic differentiation of BMSCs. Under the same conditions, the osteogenesis of miR-26a was slightly better than that of miR-218.关键词
干细胞/骨髓干细胞/聚乙烯亚胺/miR-218/miR-26a/骨髓间充质干细胞/成骨分化/RNA干扰/转染/载体/国家自然科学基金分类
医药卫生引用本文复制引用
孙璇,魏泽全,布文奂,方滕姣子,西洛片多,刘麒麟,孙宏晨..聚乙烯亚胺递送miR-218及miR-26a对骨髓间充质干细胞成骨分化的影响[J].中国组织工程研究,2017,21(25):3964-3970,7.基金项目
国家自然科学基金项目(81320108011) （81320108011）
吉林省省级产业创新专项资金项目(2016C044-3) （2016C044-3）
吉林省自然科技基金项目(20170101093JC) （20170101093JC）
大学生创新创业训练计划(2015781125) the National Natural Science Foundation of China, No. 81320108011 （2015781125）
Jilin Province Industrial Innovation Special Fund Project, No. 2016C044-3 （）
the Natural Science Foundation of Jilin Province, No. 20170101093JC （）
the Innovation and Entrepreneurship Training Plan for Undergraduates, No. 2015781125 （）
