中国组织工程研究2017，Vol.21Issue(25)：3971-3976,6.DOI:10.3969/j.issn.2095-4344.2017.25.006
年龄对猕猴骨髓间充质干细胞体外分化和细胞因子水平的影响
Age effects onin vitro differentiation and cytokine levels of macaque bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND: Mesenchymal stem cells attract extensive attention because of good biological characteristics and broad prospects, but the cells gradually show the characteristics of the aging with the increase of individual age or incubation time in vitro. Nonhuman primates have similar biological characteristics with human being, and have unique advantage in the animal model and disease treatment research.OBJECTIVE: To analyze the difference in proliferation and differentiation of bone marrow mesenchymal stem cells from macaques at different ages and to explore the effect of age on bone marrow mesenchymal stem cells and the possible mechanism.METHODS: Bone marrow samples from male macaques aged < 3 years and over 20 years were collected through bone marrow puncture, and divided into young group and elder group, with three macaques in each group. Then, bone marrow mesenchymal stem cells were isolated and cultured in vitro, and the morphological changes, proliferation and differentiation ability were observed. Age-related beta-galactosidase staining was performed, and protein microarray and ELISA were used to detect cytokine levels.RESULTS AND CONCLUSION: With age, the proliferation and differentiation of bone marrow mesenchymal stem cells from the elder macaques were reduced significantly, and the number of senescent cells increased significantly; the levels of interleukin-1b, interleukin-4, interleukin-6, tumor necrosis factor α and vascular endothelial growth factor were elevated obviously, the levels of heparin-binding basic fibroblast growth factor and placental growth factor were reduced. These findings indicate that the body's aging lead to the reduction in the proliferation, differentiation and cytokine secretion of bone marrow mesenchymal stem cells.关键词
干细胞/骨髓干细胞/骨髓间充质干细胞/衰老/猕猴/细胞鉴定/细胞因子分类
医药卫生引用本文复制引用
陈裕浩,朱向情,郭德镔,林庆铿,何志旭,潘兴华..年龄对猕猴骨髓间充质干细胞体外分化和细胞因子水平的影响[J].中国组织工程研究,2017,21(25):3971-3976,6.基金项目
云南省创新团队(2015HC020) （2015HC020）
昆明市重点项目(2015-1-S-00973) （2015-1-S-00973）
全军实验动物专项(SYD[2014]003) the Innovation Team of Yunnan Province, No. 2015HC020 （SYD[2014]003）
the Major Project of Kunming City, No.2015-1-S-00973 （）
the PLA Special Fund for Experimental Animals, No. SYDW[2014]003 （）
