新医学2017，Vol.48Issue(9)：609-612,4.DOI:10.3969/j.issn.0253-9802.2017.09.004
Cofilin-1 siRNA的设计及其对细胞自噬的影响
Knock-down of Cofilin-1 by siRNA and its effect on cellular autophagy
摘要
Abstract
Objective To design the small interfering RNA (siRNA) of Cofilin-1, and to explore the effect of Cofilin-1 siRNAs on autophagy.Methods Three pairs of siRNA targeting human Cofilin-1 were designed using online tools and according to the reported literatures.The protein expression of Cofilin-1, p62, LC3Ⅰ and LC3Ⅱ were detected by Western blot before and after HEK293 cells were transfected by Cofilin-1 siRNA.Autophagy flux (the changes of autophgosome and autolysosome) was observed by mRFP-GFP-LC3 double fluorescence.Results The expression of Cofilin-1 was knocked down by siRNA-a and siRNA-b successfully.After down-regulation of Cofilin-1 effectively, the expression of p62 was decreased, and the ratio of LC3Ⅱ/LC3Ⅰ was increased in HEK293 cells.Furthermore, autophgosome and autolysosome were enhanced.Conclusion siRNA can effectively knock down the expression of Cofilin-1;Cofilin-1 is involved in cellular autophagy process.关键词
Cofilin-1/干扰/小分子干扰RNA/细胞自噬Key words
Cofilin-1/Knock-down/Small infenfering RNA/Cellular autophagy引用本文复制引用
李爱群,黄桢钧,刘少军..Cofilin-1 siRNA的设计及其对细胞自噬的影响[J].新医学,2017,48(9):609-612,4.基金项目
广东省科技计划项目(2014A02021237,2016A020215163) （2014A02021237,2016A020215163）
