烟草科技2017，Vol.50Issue(8)：1-9,9.
烟草转录因子MYB12基因克隆及表达模式分析
Cloning and expression analysis of transcription factor gene MYB12 from Nicotiana tabacum
摘要
Abstract
R2R3-MYB family is one of the largest transcription factor families in plants, and plays an important regulatory role in plant development and metabolism. To identify the biological functions and regulation mechanism of MYB12 transcription factors in tobacco, two MYB12 genes (NtMYB12a and NtMYB12b) were cloned from Nicotiana tabacum with homologous cloning strategy, gene expression patterns were analyzed by real-time quantitative PCR (qPCR), and their functions and regulation mechanisms were predicted by bioinformatic analysis. The results showed that the nucleotide sequence and deduced amino acid sequence identities between NtMYB12a and NtMYB12b were 92.91％ and 87.91％, respectively. Highly conserved tobacco MYB12 genes included 4 exons and 3 introns. Phylogenetic analysis showed that NtMYB12a was evolved from Nicotiana tomentosiformis, while NtMYB12b from Nicotiana sylvestris. The expression patterns of NtMYB12a and NtMYB12b showed spatial and temporal specificity. Under topping, salinity stress, drought, darkness and phosphate starvation treatments, the expression levels of NtMYB12a and NtMYB12b were significantly different. The expression level of NtMYB12a in tobacco leaves presented an increase tendency after topping. Salinity stress, darkness and phosphate starvation significantly down-regulated the expression level of NtMYB12a, in comparison with no effect of drought. The expression levels of NtMYB12b were up-regulated under all treatments. The results indicated that NtMYB12 might play an important regulatory role in responding to abiotic stress.关键词
烟草/转录因子/NtMYB12/表达模式/非生物胁迫/基因克隆Key words
Tobacco/Transcription factor/NtMYB12/Expression pattern/Abiotic stress/Gene clone分类
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王姗姗,赵影影,李超杰,谢小东,武明珠,李锋,杨军,王中..烟草转录因子MYB12基因克隆及表达模式分析[J].烟草科技,2017,50(8):1-9,9.基金项目
中国烟草总公司烟草基因组计划重大专项项目"烟草转录因子基因芯片研制及重要转录因子表达调控研究"[110201401011(JY-11)]. （JY-11）
