烟草科技2017，Vol.50Issue(8)：10-15,6.
施氮量对烤烟成熟期中部烟叶碳氮代谢及相关基因表达的影响
Effects of nitrogen application rate on carbon and nitrogen metabolisms and related gene expression in middle leaves of flue-cured tobacco at mature stage
摘要
Abstract
To investigate the effects of nitrogen application rate on the carbon and nitrogen metabolisms of flue-cured tobacco, field experiments were conducted on cv. Yuyan 10. Three treatments were set as following:low nitrogen (22.5 kg/hm2), moderate nitrogen (26.25 kg/hm2) and high nitrogen (30 kg/hm2). The cell ultrastructure, variations of carbon and nitrogen metabolites and related gene expression patterns of middle leaves of flue-cured tobacco at mature stage were analyzed. The results showed that with the increase of nitrogen application rate, the starch grain volume in cells and the contents of starch and total nitrogen in tobacco leaves increased, while the contents of total sugar and reducing sugar decreased. With the growth of tobacco leaves, AGPase gene (a key gene in carbon metabolism) expression increased, but the increase rate decreased with the increase of nitrogen application rate. NR gene (a key gene in nitrogen metabolism) expression decreased with the increase of nitrogen application rate.关键词
氮肥/烤烟/细胞超微结构/碳氮代谢/基因表达Key words
Nitrogen fertilizer/Flue-cured tobacco/Cell ultrastructure/Carbon and nitrogen metabolism/Gene expression分类
轻工纺织引用本文复制引用
牛德新,连文力,崔红,杨胜男,常栋,张松涛,杨永霞,张洪映,贾宏昉..施氮量对烤烟成熟期中部烟叶碳氮代谢及相关基因表达的影响[J].烟草科技,2017,50(8):10-15,6.基金项目
中国烟草总公司特色优质烟叶开发重大专项"浓香型特色优质烟叶开发项目-浓香型特色优质烟叶形成的代谢与分子基础研究"(110200902045 TS-01). （110200902045 TS-01）
