烟草科技2017，Vol.50Issue(8)：23-29,7.
芽孢杆菌LL17的分离鉴定及其对仓储烟叶霉变菌的抑制效果
Isolation and identification of Bacillus LL17 and its inhibition effects on mildew fungi of stored tobacco
摘要
Abstract
To effectively control the mildew of stored tobacco leaf, a Bacillus strain LL17 was isolated from tobacco rhizosphere soil. A dual culture method and Oxford cup method were used to study the antagonistic effects of strain LL17 on three kinds of mildew fungi. The bacteria cultural, morphological and biochemical features were observed, and in addition 16S rDNA sequence analysis was used to identify the strain. The results showed that strain LL17 was identified as Bacillus subtilis on the taxonomy. The inhibition zones were clearly observed in PDA by using the dual culture method and Oxford cup method, indicated that strain LL17 was strongly antagonistic towards three kinds of mildew fungi. Sterile filtrate of strain LL17 was antagonistic towards 2 kinds of Aspergillus conidiospores. The sterile filtrate could affect spore germination or induce hyphae deformation. Therefore, strain LL17 might have the potential application prospect and value in biological control of tobacco mildew during storage.关键词
仓储烟叶/霉变/芽孢杆菌/鉴定/拮抗作用Key words
Stored tobacco/Mildew/Bacillus/Identification/Antagonism分类
轻工纺织引用本文复制引用
朱大恒,张鹏,马轩,朱润琪,席宇,刘丽,刘雨松,吴少雄,张可可,程方,喻锐,毛运浩,吴诗佳..芽孢杆菌LL17的分离鉴定及其对仓储烟叶霉变菌的抑制效果[J].烟草科技,2017,50(8):23-29,7.基金项目
国家级大学生创新创业训练计划资助项目"烟草废水的脱色、脱毒及发酵制备 L-乳酸的研究"(201610459070) （201610459070）
中国烟草总公司科技重点项目"烤烟高温逼熟形成机制、指标体系与防控技术研究"(110201402011). （110201402011）
