烟草科技2017，Vol.50Issue(8)：46-51,74,7.
DART-MS/MS法测定烟草及烟草制品中的烟碱
Determination of nicotine in tobacco and tobacco products by DART-MS/MS
摘要
Abstract
In order to determine nicotine in tobacco and tobacco products in real time, an online method based on Direct Analysis in Real Time (DART) ionization technique combined with mass spectrometry was established by optimizing parameters such as extraction solvent type, extraction solvent dosage and injection rate. The samples of five commercial cigarettes and five tobacco leaves were analysed by the established method, and the determined values were compared with those obtained using a continuous flow method. The results showed that: 1) Under the optimized conditions (30 mL ultrapure water as extraction solvent and sample injection rate of 0.6 mm/s), the analysis could be completed within 20 s. 2) The linear range of the method was 0.01-2.00 mg/mL with R2 of 0.9991, the recovery ranged from 98.5％ to 102.3％, and the detection limit and the limit of quantification were 6.05 and 20.17 ng/mL. 3) The relative error between DART-MS/MS method and continuous flow method was 0.24％-2.74％ . The established method is rapid, reliable and suitable for the real-time on-line determination of nicotine in tobacco and tobacco products.关键词
DART-MS/MS/烟草/烟草制品/烟碱Key words
DART-MS/MS/Tobacco/Tobacco product/Nicotine分类
轻工纺织引用本文复制引用
吴亿勤,刘秀明,秦云华,张承明,许永,杨亚玲,李超..DART-MS/MS法测定烟草及烟草制品中的烟碱[J].烟草科技,2017,50(8):46-51,74,7.基金项目
云南省应用基础青年项目"基于 DART-MS/MS 的指纹图谱技术开发"(Y0120150281) （Y0120150281）
云南烟草化学重点实验室开放基金"烟丝中烟碱及其类似物快速检测方法开发"(2016539200340111) （2016539200340111）
云南中烟工业公司重点项目"基于光谱大数据的卷烟主流烟气品质评价新技术研究"(2017JC04) （2017JC04）
云南中烟工业公司科技项目"适用于卷烟产品质量管控的分析检测平台搭建及应用"(2015JC07). （2015JC07）
