眼科新进展2017，Vol.37Issue(9)：824-827,4.DOI:10.13389/j.cnki.rao.2017.0208
树鼩茄病镰刀菌性角膜炎房水中Th1/Th2炎性因子的变化
Changes of Th1/Th2 inflammatory factors in the aqueous humor of tree shrews with fusarium solani keratitis
摘要
Abstract
Objective To observe the changes of Th1 / Th2 inflammatory factors in the aqueous humor of tree shrews with fusarium solani keratitis,as well as to explore the relationship between Thl / Th2 inflammatory factors and inflammatory response in fusarium solani keratitis.Methods Forty healthy tree shrews were randomly divided into experimental group(n =30) and control group (n =10).Fusarium solani was inoculated into sabina culture medium and cultured at 26 ℃ for 7 days,and then the fungal suspension was collected and the density of spores was adjusted to 10 × 109 CFU · mL-1.In the experimental group,50 μL fungal spore suspension was injected into the center of the cornea stroma,while the control group received the same amount of saline.Next the levels of cytokines including interleukin (IL)-1 β,IL-6,IL-4 and IL-10 were analyzed by flow cytometry on day 3,day 7,day 14 after successful modeling,and the changes in types of infiltrating cells were observed by histopathological examination.Results The expression level of IL-1 β and IL-6 (Th1 type cytokines) was the highest on day 7,and the difference was statistically significant at each time point when compared with the control group (all P ＜ 0.05).The expression level of IL-10 (Th2 type cytokines) was the highest on day 14,and the difference was statistically significant at each time point when compared with the control group (all P ＜ 0.05).The difference in IL-4 expression was statistically significant on day 7 (P ＜ 0.05).In addition,histopathological examination showed that the number of infiltration cell reached its peak on day 7,mainly neutrophils,and fungal hyphae were observed to be parallel to the matrix fibers at each time point.Conclusion The proinflammatory cytokines IL-1β and IL-6 and the anti-inflammatory cytokines IL-10 may play an important role in the molecular mechanism of inflammatory response of fusarium solani keratitis in tree shrews.关键词
树鼩/茄病镰刀菌/细胞因子Key words
tree shrews/fusarium solani/cytokines分类
医药卫生引用本文复制引用
李妍,李丽红,刘海,盛智超,闵婕,胡竹林..树鼩茄病镰刀菌性角膜炎房水中Th1/Th2炎性因子的变化[J].眼科新进展,2017,37(9):824-827,4.基金项目
云南省卫生科技计划项目(编号:2016NS182、2017NS129) （编号:2016NS182、2017NS129）
昆明医科大学博士创新基金(编号:2016D07)Yunnan Medical and Scientific Development Program (No:2016NS182,2017NS129) （编号:2016D07）
Doctor's Innovation Found of Kunming Medical University(No:2016D07) （No:2016D07）
