局解手术学杂志2017，Vol.26Issue(9)：630-634,5.DOI:10.11659/jjssx.02E017051
TGF-β微泡靶向治疗大鼠骨骼肌损伤的实验研究
Research of TGF-β microbubble targeting for skeletal muscle injury in rats
摘要
Abstract
Objective To explore the effect of TGF-β microbubble targeting on the treatment of skeletal muscle injury in rats.MethodsEighty SD rats of 2 months old were randomly divided into experimental group(including TGF-β microbubble),pure drug group,microbubble group(exclusive drug) and control group,20 SD rats in each group.Four groups accepted ultrasonic transmission with consistent time and frequency.The contraction stress and stress relaxation of injured gastrocnemius muscle were measured,the changes of injured gastrocnemius muscle were observed by light microscope and electron microscope.Results There was no statistically significant difference in contraction stress of gastrocnemius muscle among all groups at 1,5,9 days after injury(P>0.05).And at 14 days,the contraction stress of gastrocnemius muscle of the experimental group and the pure drug group were obviously higher than those of the rest two groups,the differences were significant(P<0.05).The difference of gastrocnemius muscle contraction stress was statistically significant between the experimental group and the pure drug group(P<0.05).For the stress relaxation of gastrocnemius muscle,the experimental group was lower than the other three groups at 1 day after injury,the differences were significant(P<0.05).Light microscopy showed the number of muscle fiber in the experimental group increased obviously at 9 days after injury.There was no scarring at 14 days after injury.Electron microscope showed mitochondria and sarcoplasmic reticulum increased in the experimental group,and the new muscle cells and satellite cells were significantly more than those of the rest three groups.Conclusion TGF-β cell targeted therapy can obviously improve the shrinkage stress of skeletal muscle in rats after injury and recover the stress decay,which can obviously promote the injury repair and regeneration of skeletal muscle,and reduce scar formation at the same time.关键词
转化生长因子β/骨骼肌/损伤/再生/超声Key words
TGF-β/skeletal muscle/injury/regeneration/ultrasound分类
医药卫生引用本文复制引用
王军,程少文,陈庆玉,袁伟,曾允富..TGF-β微泡靶向治疗大鼠骨骼肌损伤的实验研究[J].局解手术学杂志,2017,26(9):630-634,5.基金项目
海南省卫生厅资助课题(琼卫2013资助-038) （琼卫2013资助-038）
