局解手术学杂志2017，Vol.26Issue(9)：634-637,4.DOI:10.11659/jjssx.05E017032
三七总皂苷对大鼠局灶性脑缺血再灌注后IL-10及TNF-α表达的影响
Effects of panax notoginseng saponins on expression of IL-10 and TNF-α in rats with focal cerebral ischemia-reperfusion
摘要
Abstract
Objective To investigate the effects of panax notoginseng saponins(PNS) on the expression of IL-10 and TNF-α in the brain after focal cerebral ischemia-reperfusion injury in rats,and the mechanism was explored.Methods 30 male adult Sprague-Dawley rats were randomly divided into sham-operation group,ischemia-reperfusion group and PNS treatment group,10 rats in each group.The middle cerebral artery occlusion(MCAO) was established with ischemia-reperfusion group.The rats received continuous gastric lavage for 4 weeks,who were observed for 3 days and then killed to the detect the expression of IL-10 and TNF-αin ischemic region by ELISA,immunohistochemistry and RT-PCR.The behavioral ability score in rats were tested.Results Compared with the sham-operation group and ischemia-reperfusion group,the expression of IL-10 was increased,while the expression of TNF-α was decreased significantly in PNS treatment group,behavior score of PNS treatment group increased significantly than that of ischemia-reperfusion group.Conclusion The panax notoginseng saponins can reduce the neurons injury after focal cerebral ischemia-reperfusion injury in rats partly by regulating the expression of IL-10 and TNF-α in the brain.关键词
三七总皂苷/脑缺血/再灌注损伤/白细胞介素-10/肿瘤坏死因子Key words
panax notoginseng saponins/cerebral ischemia/reperfusion injury/IL-10/TNF-α分类
医药卫生引用本文复制引用
丁小明,许嵘..三七总皂苷对大鼠局灶性脑缺血再灌注后IL-10及TNF-α表达的影响[J].局解手术学杂志,2017,26(9):634-637,4.基金项目
2016年福建省中青年教师教育科研项目(JAT160852) （JAT160852）
