局解手术学杂志2017，Vol.26Issue(9)：638-643,6.DOI:10.11659/jjssx.11E016007
单术者制作大鼠腹腔异位心脏移植模型操作技巧
Establishment skills of a rat model of abdominal heterotopic heart transplantation by single operator
摘要
Abstract
Objective To explore the operation procudure and notice of establishing a rat model of abdominal heterotopic heart transplantation by single operator,and summarize the detailed skill and experience for beginners.Methods 68 pairs male SD(recipients)/Wistar(donors) rats,preoperative anesthesia for recipients and donors at the same time,8/0 line was preparing for blocking recipients vena cava and abdominal aortic,blocking the branch vessel.Left and right superior vena ligature respectively with hilar,cutting the ascending aorta,perfusion cardioplegia,free pulmonary artery,across transverse sinus of pericardium for pulmonary artery cutting,atrial wash,free donor heart.Choosed appropriate incision of receptor abdominal blood vessels,mattress suture at 6 and 12 points,a single suture for vent gases,continuous suture in artery,single needle in vein,reperfusion after exhaust gas.Recording operation time,HE staining,IL-1β/CD3 immunohistochemistry slice,flow cytometry analysis of CD4+T/CD8+T lymphocytes subsets in the peripheral blood.Results Sixty-eight cases were treated by single operater,the operation time was(58.8±4.5)minutes,artery suture time was(7.6±2.2)minutes,vein suture time was(13.5±4.2)minutes,the total donor heart ischemic time was(31.8±4.5)minutes,the success rate was 88.2％.The rejection reaction was stronger on the third and fifth day after surgery,with high expression of IL-1β/CD3 in cardiac allografts.The CD4+T/CD8+T lymphocytes subsets increased in the peripheral blood at first day after heart transplantation.Conclusion By fully preparation and skillfully operation,establishing a rat model of abdominal heterotopic heart transplantation by single operator has a stable success rate.关键词
心脏移植/动物模型/大鼠/外科手术/操作流程Key words
heart transplantation/animal model/rats/surgical operation/operation procudure分类
医药卫生引用本文复制引用
余春俊,王贤灿,戴仕林,吴伟,尚玉强..单术者制作大鼠腹腔异位心脏移植模型操作技巧[J].局解手术学杂志,2017,26(9):638-643,6.基金项目
新疆维吾尔自治区科技计划项目(201233149) （201233149）
