局解手术学杂志2017，Vol.26Issue(9)：643-647,5.DOI:10.11659/jjssx.01E017068
Wnt/β-catenin信号通路对哮喘大鼠气道平滑肌细胞迁移能力的影响
Role of Wnt/β-catenin signaling pathway in migration of asthmatic rat airway smooth muscle cells
张勇 1王晓芬2
作者信息
- 1. 四川现代医院呼吸内科,四川 成都 610000
- 2. 成都军区总医院重症医学科,四川 成都 610038
- 折叠
摘要
Abstract
Objective To investigate the role of Wnt/β-catenin signaling pathway in migration ability of asthmatic rat airway smooth muscle cells (ASMCs).Methods The asthmatic rat airway smooth muscle cells were cultured in vitro.The expression of Wnt/β-catenin in ASMCs was detected by RT-PCR and Western blot.Migration ability of ASMCs was detected by wound healing assay and transwell migration assay.Results Compared with normal rats,the expression level of Wnt/β-catenin in chronic asthmatic rats ASMCs was obvious higher.The β-catenin signaling pathway by specific siRNA could reduce beta-catenin mRNA and protein expression level of ASMCs.The knockdown of Wnt/β-catenin was able to decreases the migration ability of ASMCs.Conclusion Abnormal activation of Wnt/β-catenin signaling pathway may participants in the migration ability of ASMCs of chronic asthma rats.关键词
Wnt/β-catenin/哮喘/气道平滑肌细胞/迁移Key words
Wnt/β-catenin/asthma/asthmatic/airway smooth muscle cells/migration分类
医药卫生引用本文复制引用
张勇,王晓芬..Wnt/β-catenin信号通路对哮喘大鼠气道平滑肌细胞迁移能力的影响[J].局解手术学杂志,2017,26(9):643-647,5.
