中国康复理论与实践2017，Vol.23Issue(9)：1027-1031,5.DOI:10.3969/j.issn.1006-9771.2017.09.008
APP/PS1转基因小鼠学习记忆能力下降与胼胝体脱髓鞘损伤的关系
Learning and Memory Deficit and Demyelination of Corpus Callosum in APP/PS1 Transgenic Mice
摘要
Abstract
Objective To investigate the relationship between learning and memory deficit and demyelination of the corpus callosum in twelve-month old APP/PS1 transgenic mice. Methods Twelve twelve-month old APP/PS1 transgenic mice were as AD group, and age-matched wild type (WT) littermates were as WT group. Learning and memory ability was tested with Morris water maze, and the mor-phology of nerve fiber of corpus callosum was detected with Luxol Fast Blue staining. Immunohistochemistry was used to detect myelin ba-sic protein (MBP) in the corpus callosum. Thioflavine S staining was used to detect amyloid plaque in the corpus callosum. Results Com-pared with WT group, the latency increased (Z>2.873, P<0.01) and the times crossing the location of the platform decreased (t=-7.339, P<0.001) in AD group. The nerve fibers were sparse and disorganized, with a lot of vacuoles in the corpus callosum of AD group. The positive expression of MBP in the corpus callosum was significantly decreased (t=-4.481, P<0.001) in AD group compared with WT group. There were amyloid plaques in the corpus callosum of AD group. Conclusion Twelve-month old APP/PS1 transgenic mice exhibit learning and memory deficit, which may be attributed to the deposition of the amyloid plaque mediated demyelinated injury of the corpus callosum.关键词
阿尔茨海默病/学习记忆/胼胝体/脱髓鞘/小鼠Key words
Alzheimer's disease/learning and memory/corpus callosum/demyelination/mice分类
医药卫生引用本文复制引用
张秀峰,陶静,陈立典,金昊,林冰冰,李珑,宋长明,李钻芳,梁胜祥,毛敬洁,柳维林..APP/PS1转基因小鼠学习记忆能力下降与胼胝体脱髓鞘损伤的关系[J].中国康复理论与实践,2017,23(9):1027-1031,5.基金项目
1.福建省康复技术协同创新中心课题(No.X2016001-协同) （No.X2016001-协同）
2.福建中医药大学科研创新平台专项(No.X2015024-平台). （No.X2015024-平台）
