中国康复理论与实践2017，Vol.23Issue(9)：1032-1036,5.DOI:10.3969/j.issn.1006-9771.2017.09.009
沉默ski基因对大鼠星形胶质细胞活化增殖及Cyclin D1表达的影响
Effects of Knocking Down ski on Proliferation of Astrocytes and Expression of Cyclin D1 in Rats
摘要
Abstract
Objective To investigate the role of ski in proliferation of astrocytes and the molecular mechanisms in rats. Methods Astro-cytes were obtained from cerebral cortex of a three-day old rat and cultured in vitro. siRNA targeted to ski and negative control sequences were prepared. The astrocytes were divided into ski-siRNA group, siRNA negative control group and untreated control group, while the spe-cific siRNA targeting ski negative control sequences were transfected into astrocytes with Lipofectamine? RNAiMAX Reagent. The protein levels of ski, glial fibrillary acidic protein (GFAP) and Cyclin D1 were determined with Western blotting. The proliferation of astrocytes were measured with CCK8 assay. The cell-cycle of astrocytes were analyzed with flow cytometer. Results The protein level of ski (F=38.611, P<0.01), GFAP (F=7.547, P<0.05) and Cyclin D1 (F=3.901, P<0.05) reduced in ski-siRNA group, the proliferation of astrocyte was significantly inhibited since twelve hours after culture (F>30.507, P<0.01), and less cells were in S phase and more in G1/G0 phase (F>48.425, P<0.01), compared with the control groups. Conclusion ski knocking down by siRNA significantly inhibits the proliferation of astro-cytes, which may associate with the down-regulation of Cyclin D1 expression.关键词
ski/RNA干扰/增殖/星形胶质细胞/大鼠Key words
ski/small interfering RNA/proliferation/astrocyte/rats分类
医药卫生引用本文复制引用
赵鑫,郭永强,寇江力,丁宁,周开升,南伟,张海鸿..沉默ski基因对大鼠星形胶质细胞活化增殖及Cyclin D1表达的影响[J].中国康复理论与实践,2017,23(9):1032-1036,5.基金项目
1.国家自然科学基金项目(No.30772299) （No.30772299）
2.兰州大学第二医院科研基金项目(No.sdkyjj-04). （No.sdkyjj-04）
