中国康复理论与实践2017，Vol.23Issue(9)：1051-1055,5.DOI:10.3969/j.issn.1006-9771.2017.09.012
体外培养大鼠星形胶质细胞水通道蛋白4的表达
Expression of Aquaporin 4 in Astrocytes of Rats in Vitro
摘要
Abstract
Objective To explore the expression of aquaporin 4 (AQP4) in primary and secondary cultured rat astrocytes. Methods Rat cortical astrocytes from a newborn (one day) Wistar rat were cultured. Astrocytes were identified with immunofluorescence staining of glial fibrilillary acidic protein (GFAP). The expression of AQP4 was determined with real-time quantitative polymerase chain reaction and immu-nofluorescence staining three, five, seven and nine days of primary culture, and nine days of secondary culture. Results The purity of GFAP-positive cells was more than 95％. The expression of AQP4 mRNA was found three days of primary culture, remained unchanged five days of primary culture (P>0.05), and increased seven and nine days of primary culture (P<0.05). The expression of AQP4 mRNA was not different between nine days of primary culture and nine days of secondary culture (P>0.05). AQP4 immunofluorescence staining showed the same trend of AQP4 mRNA. Conclusion AQP4 may express since three days of primary culture in rat astrocytes in vitro, and increase slowly until nine days of primary culture.关键词
水通道蛋白4/星形胶质细胞/细胞培养/大鼠Key words
aquaporin 4/astrocyte/cell culture/rats分类
医药卫生引用本文复制引用
徐立新,董丽萍,师忠芳,闫旭,杨少华,袁芳..体外培养大鼠星形胶质细胞水通道蛋白4的表达[J].中国康复理论与实践,2017,23(9):1051-1055,5.基金项目
1.国家自然科学基金项目(No. 81271286) （No. 81271286）
2.北京市自然科学基金项目(No. 7152027). （No. 7152027）
