中国临床医学2017，Vol.24Issue(4)：514-518,5.DOI:10.12025/j.issn.1008-6358.2017.20170621
肝细胞肝癌肿瘤微环境中功能耗竭CD8+T细胞脂代谢特征的观察
Lipid metabolic characteristics of functionally exhausted CD8+T cells in tumor microenvironment of hepatocellular carcinoma
摘要
Abstract
Objective:To explore the lipid metabolic characteristics of functionally exhausted CD8+T cells in tumor microenvironment of hepatocellular carcinoma(HCC).Methods:By using flow cytometry analysis and real-time PCR to detect the functions and lipid metabolism-related proteins and genes expression of CD8+T cells in HCC and peri-tumor tissues,then the relationship between function and lipid metabolism was analyzed.Objective:The infiltrated CD8+T cells in HCC have the following characteristics: functional exhaustion(decreased interferon-γ secretion and up regulated programmed death molecule-1 expression);down regulated expression of multiple fatty acid synthases,and the decreased content of intracellular fatty acids;impaired mitochondrial structure and function(P<0.05).Conclusions:Fatty acid metabolism abnormalities are the characteristics of exhausted CD8+T cell infiltrated in HCC.关键词
肝细胞肝癌/CD8+T细胞/代谢/功能耗竭/微环境Key words
hepatocellular carcinoma/CD8+T cells/metabolism/functional exhaustion/microenvironment分类
医药卫生引用本文复制引用
沈琦,卓乐盈,刘芳铭,张苗苗,王晗,刘卫仁,武多娇..肝细胞肝癌肿瘤微环境中功能耗竭CD8+T细胞脂代谢特征的观察[J].中国临床医学,2017,24(4):514-518,5.基金项目
上海市浦江人才计划(17PJD006),上海市卫计委科研项目(201740101).Supported by Pujiang Talent Plan of Shanghai (17PJD006) and Project of Shanghai Municipal Health Planning Commission (201740101). （17PJD006）
