中国临床医学2017，Vol.24Issue(4)：519-524,6.DOI:10.12025/j.issn.1008-6358.2017.20170616
信号转导及转录激活蛋白4对脂多糖所致小鼠急性肺损伤的影响
Effect of STAT4 on LPS induced acute lung injury of mice
摘要
Abstract
Objective:To discuss the effect of signal transducer and activator of transcription 4(STAT4)in bacteria-lipopolysaccharide(LPS)induced acute lung injury(ALI)on mice.Methods:Mice with gene STAT4 knocked out(STAT4-/-)were used.In the models of LPS induced ALI model,arterial blood gas analysis was utilized,and change of myeloid-derived suppressor cells(MDSCs)and regulator CD4+CD25+Treg cells were observed using flow cytometry.Pulmonary inflammatory cells were analyzed by Wright stain.Objective:In the models of LPS induced ALI,lung injury was alleviated in STAT4-/-mice compared to wild type(WT)mice.PaO2/FiO2 was higher,histological score was lower,lung wet/dry ratio decreased,MDSCs and CD4+CD25+Treg cells were increased,total number of inflammatory cells and neutrophils were higher in STAT4-/-mice compared to WT mice(P<0.05).Conclusions:Deficiency of STAT4 could alleviate LPS induced ALI.The potential mechanism may be associated with increased ratios of MDSCs and CD4+CD25+Treg cells in the blood.关键词
信号转导及转录激活蛋白4/脂多糖/肺损伤Key words
signal transducer and activator of transcription 4/lipopolysaccharide/lung injury分类
医药卫生引用本文复制引用
刘歆,吴晓丹..信号转导及转录激活蛋白4对脂多糖所致小鼠急性肺损伤的影响[J].中国临床医学,2017,24(4):519-524,6.基金项目
国家自然科学基金(81500058),上海市卫生和计划生育委员会课题(15GWZK0102),上海市公共卫生三年行动计划重点学科建设项目传染病与卫生微生物学(2016/01-2017/12),福建省自然科学基金面上项目(2017J01140).Supported by National Natural Science Foundation of Youth Science Fund Project (81500058),Shanghai Municipal Health and Family Planning Commission (15GWZK0102),Shanghai City Public Health Three-Year Action Plan for Key Discipline Construction of Infectious Diseases and Health Microbiology (2016/01-2017/12),and Natural Science Foundation Project of Fujian Province (2017J01140). （81500058）
