中国输血杂志2017，Vol.30Issue(7)：762-766,5.DOI:10.13303/j.cjbt.issn.1004-549x.2017.07.038
华东地区献血者人群隐匿性乙型肝炎病毒感染及S区变异分析
Study on prevalence and mutation of amino acid sequence in S gene of OBI blood donors in three blood centers in China
蒋菲菲 1吕蓉 1李敏 2赵阳 1林洪铿 3钱榕4
作者信息
- 1. 安徽省血液中心,安徽合肥230031
- 2. 安徽医科大学第四附属医院
- 3. 福建省血液中心
- 4. 江西省血液中心
- 折叠
摘要
Abstract
Objective To study the prevalence of the occult hepatitis B virus infection (OBI) and the mutation of amino acid sequence in S gene of voluntary blood donors in AnHui/FuJian/Jiang Xi Province Blood centers.Methods Serologic testing for anti-HBc by ELISA was performed with HBsAg-HBV DNA+ samples from voluntary blood donors in three province blood centers.The S region of HBV of those samples was amplified and sequenced.The genotype and mutation of amino acid sequence were analyzed by MEGA6.Results 21 in 123046 blood donors from AnHui Province blood center were HBsAgHBV DNA+,the prevalence of OBI was 0.017％,and 76.2％ of these-OBI samples was positive in anti-HBc,S region was amplified by nest-PCR in 15 OBI samples,8 of them were B genotype,the others were C genotype.39 samples of 51 OBI blood donors from FuJian Province blood center were anti-HBc positive,16 samples of those OBI donors were amplified S region,14 were B genotype,the others were C genotype.There are 30 OBI blood donors from JiangXi Province blood center,24 of them were anti-HBc positive,S region was amplified in 4 samples,1 was B genotype,the others were C genotype.Of all 35 OBI samples,26 showed amino acid mutation,which was in MHR region of S gene,especially in HBV "α" epitope.Conclusion The rate of prevalence of OBI in AnHui Province was 0.017％,there was also certain OBI infection in FuJian and JiangXi Province.In the OBI samples which were amplified S region,the positive rates of anti-HBc in three blood centers were 73.3％,93.8％,100％.B Genotype was the main HBV genotype.The mutation in MHR region of S gene,especially in HBV "α" epitope,may be one of the reasons to cause OBI.关键词
献血者/隐匿性乙型肝炎病毒感染(OBI)/S区/变异Key words
Blood donor/Occult hepatitis B virus infection (OBI)/S gene/Mutation分类
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蒋菲菲,吕蓉,李敏,赵阳,林洪铿,钱榕..华东地区献血者人群隐匿性乙型肝炎病毒感染及S区变异分析[J].中国输血杂志,2017,30(7):762-766,5.
