中国输血杂志2017，Vol.30Issue(7)：780-784,5.DOI:10.13303/j.cjbt.issn.1004-549x.2017.07.045
嘉兴地区献血人群隐匿性乙肝病毒感染血清学及病毒学特征研究
Serological and virological epidemiology characterization of occult hepatitis B virus infection in Jiaxing volunteer blood donors
摘要
Abstract
Objective To research and analyze serological and virological epidemiology charactererization of occult hepatitis B virus infection in Jiaxing volunteer blood donors.Methods 52 698 samples were screened by ELISA(HBsAg、antiHCV 、anti-HIV、anti-TP) and Nucleic acid amplification technique(NAT),then NAT positive samples were further identified to detect virus type.HBsAg-/HBV-DNA+ samples were collected in three different kinds of qualitative HBsAg detection of ELISA kit.The quantitative determination of HBsAg and anti-HBs were used by chemiluminescencemethod.At the same time,real-time fluorescence quantitative PCR (QPCR) was used to measure the viral load of HBV.Further analysis and study on the serological and virological distribution of OBI combined with five markers of hepatitis B virus (HBV),with tracing general epidemiological data (sex,age and age).Results The prevalence rate of OBI was 0.89‰ (1 ∶ 1 121) in all donors with OBI infection,and 2 cases of window period (WP) were found in 52698 donors (1 ∶ 26 349).The results of HBsAg and HBeAg were negative in 49 HBsAg-/HBV-DNA+ samples,and 6OBI serological profiles were found.Anti-HBs quantitative concentration(＞100 mIU/mL)accounted for 27.66％ (13/47),while anti-HBc+ positive rate was 91.49％ (43/47).HBV-DNA nucleic acid quantitative ranged from 4.10 to 1.82× 103(IU/mL) (median of 15.83),whereas HBsAg+/HBV-DNA+positive viral load was in the range of 61.47 to 1.28× 104(IU/mL) (median of 538.15).The difference was significant in viral load between experiment group and control group(P＜0.05).Male donors of more than 40 years were higher in prevalence rate of OBI infection (P＜0.05),meanwhile there was a significant difference in OBI infection rate between repeated blood donors and fnrst blood donors(0.01＜P＜0.05).Conclusion The viral load was low in OBI infected donors,and anti-HBc+ was the main manifestation.NAT had the ability to detect OBI,shorten the window period,and contributed to ensure the safety of clinical blood.关键词
隐匿性乙型肝炎病毒感染/HBsAg/抗-HBc/献血者/核酸扩增技术Key words
occult hepatitis B virus infection/HBsAg/Anti-HBc/blood donors/nucleic acid amplification technique分类
临床医学引用本文复制引用
倪修文,徐利强,李建华,孙亚云,毛惠娜,吴瑾惠..嘉兴地区献血人群隐匿性乙肝病毒感染血清学及病毒学特征研究[J].中国输血杂志,2017,30(7):780-784,5.基金项目
嘉兴市社会发展领域研究与示范应用项目(2016AY23097) （2016AY23097）
