中国比较医学杂志2017，Vol.27Issue(9)：7-10,4.DOI:10.3969.j.issn.1671-7856.2017.09.002
DBA/1J小鼠胶原诱导关节炎模型的建立与鉴定
Establishment and characterization of a DBA/1 J mouse model of collagen?induced arthritis
摘要
Abstract
Objective To establish a DBA/1J mouse model of collagen?induced arthritis (CIA) and to character?ize the pathological and immunological changes of the model. Methods Bovine typeⅡcollagen ( CⅡ) was emulsified in complete Freund's adjuvant (CFA), subcutaneously injected at the root of tail of DBA/1J mice for primary immunization, and the injection was repeated 21 days later for secondary immunization. The changes of body weight, degree of joint swell?ing and other morphological indicators of arthritis were observed and pathological changes in the ankle joints were analyzed. The serum levels of inflammatory cytokines were detected by cytometric bead assay ( CBA) . Results The body weight of the CIA mice began to decrease 35 days after primary immunization and was continuously lower than that in the control group. 28 days after the primary immunization, their paws began to tern red and swollen, and after 35 days, the thickness of swol?len paws of the CIA mice was significantly greater than that in the control group (P < 0. 05). The incidence rate of CIA reached 90％ on the 62nd day. Pathological examination showed a narrowed articular space, hyperplasia of synovial tissue, inflammatory cell infiltration and varying degrees of cartilaginous damage. Compared with the control group, the serum lev?els of inflammatory cytokines IL?6, IFN?γand MCP?1 were significantly increased in the CIA mice (P< 0. 05), while the anti?inflammatory cytokine IL?10 was slightly decreased ( P> 0. 05 ) . Conclusions A collagen?induced arthritis model can be successfully established by subcutaneous injection of bovine collagen typeⅡemulsified in complete Freund' s adju?vant at the root of tail of DBA/1J mice, and provides a useful experimental tool for further studies of pathogenesis of and drug development for rheumatoid arthritis.关键词
DBA/1J小鼠/牛Ⅱ型胶原/胶原诱导性关节炎模型/炎症因子Key words
DBA/1J mice/Bovine type Ⅱ collagen/Collagen⁃induced arthritis/Inflammatory cytokines分类
医药卫生引用本文复制引用
徐慧雯,郝文婷,陈德生,张雅文,陆云娟,李向阳,秦苏萍,潘伟..DBA/1J小鼠胶原诱导关节炎模型的建立与鉴定[J].中国比较医学杂志,2017,27(9):7-10,4.基金项目
国家自然科学基金(编号:81501762) （编号:81501762）
大学生创新创业训练计划项目基金(编号:201510313017) （编号:201510313017）
江苏省博士后科研资助计划(编号:1501061A) （编号:1501061A）
江苏高校品牌专业建设工程一期项目(编号:PPZY2015B161). （编号:PPZY2015B161）
