中国比较医学杂志2017，Vol.27Issue(9)：36-40,5.DOI:10.3969.j.issn.1671-7856.2017.09.007
基于D⁃最优设计的SD大鼠皮肤化学脱毛参数优化
Optimization of chemical hair removal in SD rats by D?optimal design
摘要
Abstract
Objective To screen the optimal parameters for depilation in SD rats, and to provide an experimental prerequisite for the establishment of animal models of skin photoaging. Methods The effects of concentrations and time of the chemical depilatory Na2 S treatment on the hair removal efficiency were evaluated by the number of residual hair per milimeter square and the damaged area of the skin in rats, and the parameters for hair removal were further optimized by D?optimal design. Results Working concentration of Na2 S was the critical factor affecting its capability of hair removal. No depilation was observed when the concentration of Na2 S was lower than 5％, while skin damage began to appear and be?came severe gradually when the concentration of Na2 S was above 7％. Therefore, 6％ Na2 S was taken as the basic concen?tration for depilation. Hair removal was observed with 6％ Na2 S treatment for 5 min. After 10 min of treatment, the surface of the skin turned to be smooth, and this tendency continued until 15 min. After 15 min, skin damage occurred and turned more severe after 20 min. When treated for over 25 min, skin damage similar to skin burns was observed with naked eyes. Overall, our study with the D?optimal design showed that the effect of hair removal in the SD rats was optimal when treated with 7％ Na2 S for 10 min, causing no skin damage and residual hair left. Conclusions Highly efficient hair removal in SD rats can be achieved with chemical depilatory optimized by D?optimal design.关键词
SD大鼠/皮肤脱毛/D⁃最优设计Key words
SD rats/Hair removal/D⁃optimal design分类
医药卫生引用本文复制引用
许雅琦,徐德峰,赵子建,马忠华,赵谋明,张婷,苏国万,段军毅..基于D⁃最优设计的SD大鼠皮肤化学脱毛参数优化[J].中国比较医学杂志,2017,27(9):36-40,5.基金项目
中国博士后科学基金项目(编号:2014M552203) （编号:2014M552203）
中央高校基本科研业务费专项资金资助(编号:20152M062) （编号:20152M062）
国家级大学生创新课题(编号:20161056601). （编号:20161056601）
