中国比较医学杂志2017，Vol.27Issue(9)：48-52,5.DOI:10.3969.j.issn.1671-7856.2017.09.009
静脉注射大肠杆菌制备高动力型感染性休克犬模型
Establishment of a dog model of hyperdynamic septic shock by intravenous injection of Escherichia coli
摘要
Abstract
Objective To establish a dog model of hyperdynamic septic shock by intravenous injection of Esche?richia coli. Methods Twenty?two hybrid dogs were randomly divided into control group (n=11) and experimental group ( n=11 ) . A double?cavity central venous catheter was inserted into the right femoral vein of the dogs after anesthesia. Physiological saline was continuously pumped into the femoral vein of the dogs in the control group by the micro?pump, while the dogs of the septic shock group were pumped with E. coli in the same way. The catheter of PICCO ( pulse indicator continuous cardiac output) was inserted into the right femoral artery and the systemic hemodynamics of the dogs was moni?tored at all of the time points from 0 to 48 h. Results Compared with the control group, the heart rate (HR), cardiac output (CO) and stroke volume (SV) of the experimental group were significantly increased at 12 h (P < 0. 05), and the systolic blood pressure (SBP) and systemic vascular resistance (SVR) were significantly decreased at 12 h (P < 0. 05). However, other hemodynamic parameters such as the central venous pressure ( CVP) , the pulmonary artery wedge pressure ( PAWP) , the oxygen delivery rate ( DO2 ) , the oxygen consumption rate ( VO2 ) , and the oxygen extraction rate ( O2 ER) were not significantly different ( P > 0. 05 ) . Moreover, significant decrease in urine output was observed in the septic shock group since 12 hours, and the peak systolic velocity ( PSV) and the resistance index ( RI) of the systolic artery were significantly different from those in the control group (P < 0. 05). Conclusions The dog model of hyperdynammic septic shock can be successfully established by intravenous injection of Escherichia coli into the femoral vein by a micro?pump. This model provides an experimental basis for further study of septic shock.关键词
感染性休克/动物实验/犬/大肠杆菌/血流动力学Key words
Septic shock/Animal experiments/Canine/Escherichia coli/Hemodynamics分类
医药卫生引用本文复制引用
崔嵩,韩叶叶,刘思伯..静脉注射大肠杆菌制备高动力型感染性休克犬模型[J].中国比较医学杂志,2017,27(9):48-52,5.基金项目
大连市卫计委2013年课题项目(课题名称:《亚低温治疗感染性休克所致ARDS的实验研究》). （课题名称:《亚低温治疗感染性休克所致ARDS的实验研究》）
