中国比较医学杂志2017，Vol.27Issue(9)：60-64,5.DOI:10.3969.j.issn.1671-7856.2017.09.011
肺癌人源性肿瘤组织异种移植模型的组织学变化及其p63、napsin A和TTF⁃1的表达差异
Histological changes and expression levels of p63 , napsin A and TTF?1 in lung cancers and their patient?derived tumor xenograft models in nude mice
方天 1黄海荣 2程熙 2胡文娟 1刘彪 1许龙祥 1董敏 1恽时锋1
作者信息
- 1. 南京军区南京总医院比较医学科,南京 210002
- 2. 南京军区南京总医院心胸外科,南京 210002
- 折叠
摘要
Abstract
Objective To establish patient?derived tumor xenograft ( PDX) models of lung cancer in nude mice, and test the changes of histomorphological architecture and the expression levels of p63, napsin A and TTF?1 in the primary lung cancer tissues and the tissues of third?generation (F3) PDX models. Methods Tissue pieces of surgical specimens from 12 lung cancer patients(8 squamous cell carcinomas and 4 adenocarcinomas)were taken and subcutaneously engrafted into nude mice to establish PDX models. Samples of the primary lung cancer tissues from patients and its PDX model in mice after the F3 generation were examined by pathology using HE staining. The changes of expression levels of p63, nap? sin A and TTF?1 were detected by immunohistochemistry. Results A total of 5 cases of the F3 PDX models were estab?lished successfully, including 4 cases of squamous cell carcinoma (SCC) and one case of adenocarcinoma (ADC). The tissue samples of the PDX model showed the same histological features of the primary tumor tissues. The p63 protein was positively expressed in 84. 3％ of the SCC tissues and 96％ of the PDX models, showing a non?significant difference ( P >0. 05), while negatively expressed in the ADC tissues. The positive rate of napsin A protein expression was 66％ of the ADC tissues and 72. 4％ of the F3 PDX models, without a significant difference between them (P > 0. 05). The express?ing rate of TTF?1 protein was 91％ of the ADC tissues and 85％ of the F3 PDX models, without a significant difference ( P> 0. 05) as well. In addition, both napsin A and TTF?1 were negatively expressed in the SCC tissues. Conclusions The PDX models of lung cancers we have established retained some of the key features of the primary cancers, such as histologi?cal architecture, genomic signature, cellular heterogeneity and drug responsiveness, providing an effective resource for the research and development of personalized screening and evaluating of the drug efficacy in clinical trials and the identifica?tion of biomarkers for drug responsiveness.关键词
PDX模型/肺癌/p63/NapsinA/TTF⁃1Key words
Patient⁃derived tumor xenograft model/PDX model/Lung cancers/p63/Napsin A/TTF⁃1分类
医药卫生引用本文复制引用
方天,黄海荣,程熙,胡文娟,刘彪,许龙祥,董敏,恽时锋..肺癌人源性肿瘤组织异种移植模型的组织学变化及其p63、napsin A和TTF⁃1的表达差异[J].中国比较医学杂志,2017,27(9):60-64,5.
