中国比较医学杂志2017，Vol.27Issue(9)：76-79,4.DOI:10.3969.j.issn.1671-7856.2017.09.014
负压屏障设施动物饲养笼具的选择及其感染风险的识别
Selection of laboratory animal cages and recognition of infection risk in the negative?pressure barrier facility
摘要
Abstract
Objective Most of the airborne pathogens of infectious animal experiments in biosafety laboratories can be effectively controlled by barrier apparatus. However,the requirements for biosafety parameters of the rodent cages, which are commonly?used devices, are not clear yet. The purpose of this study is to estimate the risk of pathogen exposure by mimicking infectious animal experiments using open cage or sealed cage for rodents in barrier facility under negative pressure in our laboratory, and to evaluate the risk?controlling level of the hardware conditions and management measures of our facility. Methods E. coli ( DH5α, with the kanamycin?resistance gene, Kan+) was used as the indicator microor?ganism. The risk of pathogen exposure of the following operations were tested: mice raised in open cage or sealed cage ( without pressure gradient inside and outside) , and sealed?cage changing of mice in biosafety cabinet. The quantities of in?dicator microorganisms inside and outside the facility with negative pressure were monitored to estimate the efficiency of bio? safety?control over the facility. Results There was a risk of pathogen exposure when open cage was used in the barrier fa?cility under negative pressure, and the spread of pathogens could be controlled in barrier facility under negative pressure to some extent. There was no indicator microorganism detected when animals were raised in sealed cage without pressure gra?dient inside and outside, and the risk of pathogen exposure during the procedure of sealed?cage changing in the biosafety cabinet could be effectively controlled. Conclusions The biosafety of animal experiments can be ensured using sealed cage in barrier facility under negative pressure, even without pressure gradient inside and outside of the cage.关键词
负压屏障设施/动物实验/感染风险/密闭笼盒Key words
Negative⁃pressure barrier facility/Animal experiments/Infection risk/Sealed cage分类
医药卫生引用本文复制引用
向志光,佟巍,谷有刚,李欣悦,秦川..负压屏障设施动物饲养笼具的选择及其感染风险的识别[J].中国比较医学杂志,2017,27(9):76-79,4.基金项目
卫计委公益性行业科研专项(编号:201302006) （编号:201302006）
国家重点研发计划(编号:2016YFD0501000). （编号:2016YFD0501000）
