改良穿刺法建立大鼠无血预充非停跳体外循环后认知功能障碍模型OACSTPCD
Establishment of rats' cardiopulmonary bypass POCD model by meliorated puncture method
目的 寻找建立大鼠无血预充非停跳体外循环术后认知功能障碍模型的简便方法.方法 SD大鼠 20只,0.2％戊巴比妥钠溶液腹腔注射麻醉.经由锁骨上入路右上腔静脉穿刺引流,尾动脉穿刺灌注,隐动脉穿刺监测血压.术中持续监测心率、血氧饱和度及肛温.转流流量维持在100 ml/(min·kg),转流中根据血气结果适当补充葡萄糖酸钙、潘南金(门冬氨酸钾镁注射液)和碳酸氢钠调整血气指标,体外循环转流60 min.结果 16只大鼠成功存活,存活大鼠术后一天均可完成…查看全部>>
Objective To explore a convenient method of cardiopulmonary bypass POCD model in rats.Methods Twenty Sprague Dawley rats were anesthetized with Pelltobarbitalum Natricum i.p.Right upper vena cava was punctured for the drainage of blood and caudal artery for perfusion.Heart rate,anal temperature and blood oxygen saturation were monitored.Meanwhile,the blood gas indexes were measured and adjusted during the operation.The flow of CPB was maintained 100 ml…查看全部>>
段铭杰;孙莹杰;郑翔;李思沁;屈鹏霞
50014 济南,山东省千佛山医院
静脉穿刺认知功能障碍体外循环水迷宫
Vein puncturePocdCardiopulmonary bypassWater maze
《中国体外循环杂志》 2017 (3)
胆碱能抗炎通路介导K阿片受体激动剂在体外循环促发术后认知功能障碍中的保护作用与机制
179-184,6
国家自然科学基金资助项目(81471121)
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