中国烟草学报2017，Vol.23Issue(4)：64-69,6.DOI:10.16472/j.chinatobacco.2016.286
茉莉酸甲酯诱导烟草抗虫的有效浓度和持效期
Effective concentration and period of methyl jasmonate for inducing tobacco resistance to insect pests
摘要
Abstract
To gain insights into the mechanism of tobacco resistance to insect pests induced by methyl jasmonate (MeJA),tobacco plants were sprayed with 50μmol/L and 150μ,mol/L of MeJA.Tobacco leaves were picked at 24h and 48h after tobacco plants were treated with MeJA,and Helicoverpa armigera and Spodoptera exigua were respectively fed with tobacco leaves.The feeding amount and weight gains of H.armigera and S.exigua were determined at an interval of 6h.Subsequently,the effects of various concentration of MeJA on total polyphenol content were determined by folin-ciocalteu colorimetry and glandular hair density of tobacco leaves was investigated by histochemical staining.Results showed that (1) The feeding amount and weight gains ofH.armigera significantly decreased compared with controls within 48h after tobacco plants were sprayed with 50μmol/L MeJA or 150μmoFL MeJA.As for another test insect,S.exigua,only tobacco plants exhibited resistance within 24 h after treatments with 150μmol/L of MeJA.(2) The total polyphenol content of tobacco leaves increased when tobacco plants were sprayed with MeJA,and the increased amount of the total polyphenol content was positively correlated with the applied concentration of MeJA.(3) The glandular hair density of tobacco leaves decreased when tobacco plants were treated with MeJA,and the glandular hair density was negatively correlated with the applied concentration of MeJA.Results indicated that the inducement of MeJA on resistance of tobacco plants to insect pests depended on the concentration and exposure time of MeJA,and that the increase of total polyphenol content and changes of metabolism of tobacco plants might result in insect resistance of tobacco plants induced by MeJA.关键词
烟草/抗性/茉莉酸甲酯/持效期/有效浓度Key words
tobacco/resistance/methyl jasmonate/period/effective concentration引用本文复制引用
张林娜,游秀峰,刘向阳,陈文峰,范锐,周琳,尹新明,冯超,赵特,高飞,孙淑君..茉莉酸甲酯诱导烟草抗虫的有效浓度和持效期[J].中国烟草学报,2017,23(4):64-69,6.基金项目
河南省烟草公司科技项目专项(201205) （201205）
中国烟草总公司重点项目(110201202003) （110201202003）
