中国烟草学报2017，Vol.23Issue(4)：70-76,7.DOI:10.16472/j.chinatobacco.2016.431
肉桂醛对烟草疫霉菌的体外抑制作用
Inhibition effect of cinnamaldehyde against Phytophthora nicotiance in vitro
摘要
Abstract
[Purpose] The aim of current study was to explore the inhibitory effects of Cinnamaldehyde (CA) on Phytophthora nicotiance in vitro.[Methods] P.nicotiance was used in this study.The effects of CA were investigated by measuring mycelial radial growth,mycelia membrane permeability,reactive oxygen species (ROS) and propidium (PI) fluorescence staining,malondialdehyde (MDA) and glycerol content.[Results] (1) CA efficiently inhibited the P.nicotiance mycelial radial growth and destroyed mycelial morphology,with an EC50 value of 0.93mmol/L.(2) CA increased mycelial ROS level and cell death incidence in a dose-dependent manner,according to ROS and PI staining results.(3) The concentration increase in CA treatment led to the increment of mycelia membrane permeability and the content of mycelial MDA and glycerol.[Conclusion] CA may exhibit an antifungal effect on P.nicotiance through increasing ROS level,inducing lipid peroxidation,stimulating MDA production,damaging the mycelia membrane,and leading to cell death.关键词
肉桂醛/烟草疫霉菌/活性氧/细胞膜/抑菌Key words
cinnamaldehyde/Phytophthora nicotiance/ROS/cell membrane/antifungal引用本文复制引用
凌天孝,叶协锋,陈健,薛延丰,周涵君,张晓帆,付仲毅,秦燚鹤,马静,韩秋静..肉桂醛对烟草疫霉菌的体外抑制作用[J].中国烟草学报,2017,23(4):70-76,7.基金项目
烟草行业烟草栽培重点实验室资助项目(30800665),重庆市烟草公司“云烟品牌导向型生态优质烟叶生产技术模式构建研究与推广”(NY20140401070010) （30800665）
