中国药房2017，Vol.28Issue(24)：3400-3402,3.DOI:10.6039/j.issn.1001-0408.2017.24.25
HPLC法同时测定补骨脂药材中补骨脂二氢黄酮和补骨脂酚的含量
Simultaneous Determination of Bavachin and Bakuchiol in Psorslea corylifolia by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for content determination of bavachin and bakuchiol in Psoralea corylifolia.METHODS:HPLC method was adopted.The determination was performed on Shim-Pack VP-ODS column with mobile phase consisted of acetonitrile-water (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 246 nm,the column temperature was 30 ℃,and the sample size was 10 μL.RESULTS:The linear ranges of bavachin and bakuchiol were 48.9-489 ng(r=0.999 8),784-7 840 ng(r=0.999 9).RSDs of precision,stability and reproducibility tests were all lower than 2.0％.Average recoveries ranged 95.30％-99.63％ (RSD=1.45％,n=9),96.89％-100.82％ (RSD=1.36％,n=9).CONCLUSIONS:The method is simple,accurate,stable and reproducible,and can be used for the simultaneous determination of bavachin and bakuchiol in P corylifolia.关键词
补骨脂/补骨脂二氢黄酮/补骨脂酚/含量测定/高效液相色谱法Key words
Psoralea corylifolia/Bavachin/Bakuchiol/Content determination/HPLC分类
医药卫生引用本文复制引用
黄晓婧,周晓英,文永盛..HPLC法同时测定补骨脂药材中补骨脂二氢黄酮和补骨脂酚的含量[J].中国药房,2017,28(24):3400-3402,3.基金项目
国家药品标准提高暨2015年版药典科研项目 （）
广东省科技计划项目(No.2010B030700002) （No.2010B030700002）
