中国药房2017，Vol.28Issue(24)：3405-3408,4.DOI:10.6039/j.issn.1001-0408.2017.24.27
HPLC法同时测定小儿化毒散中3种成分的含量
Simultaneous Determination of 3 Components in Xiaoer Huadu Powder by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of paeoniflorin,berberine hydrochloride and ammonium glycyrrhizinate in Xiaoer huadu powder.METHODS:HPLC method was adopted.The separation was performed on Waters SunFireTM-C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 238 nm,and column temperature was 25 ℃.The sample size was 10 μL.RESULTS:The linear ranges of paeoniflorin,berberine hydrochloride and ammonium glycyrrhizinate were 8.808-88.08 μg/mL(r=0.999 8),1.778-17.78 μg/mL(r=0.999 6),2.533-25.33 μg/mL(r=0.999 9),respectively.LOQ were 4.404,0.889,2.533 μg/mL;LOD were 1.101,0.445,1.267 μg/mL.RSDs of precision,stability and reproducibility tests were all lower than 2％.The recoveries were 95.08％-99.61％ (RSD=1.77％,n =9),96.93％-99.94％ (RSD=0.92％,n=9),98.33％-102.05％ (RSD=1.27％,n=9).CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for simultaneous determination of paeoniflorin,berberine hydrochloride and ammonium glycyrrhizinate in Xiaoer huadu powder.关键词
小儿化毒散/高效液相色谱法/芍药苷/盐酸小檗碱/甘草酸铵Key words
Xiaoer huadu powder/HPLC/Paeoniflorin/Berberine hydrochloride/Ammonium glycyrrhizinate分类
医药卫生引用本文复制引用
曾桢,艾光丽,李婷婷,文永盛,周世玉..HPLC法同时测定小儿化毒散中3种成分的含量[J].中国药房,2017,28(24):3405-3408,4.基金项目
国家药品标准提高暨2015年版药典科研项目 （）
成都市食品药品监督管理局项目(No.201703) （No.201703）
