中国药房2017，Vol.28Issue(24)：3408-3411,4.DOI:10.6039/j.issn.1001-0408.2017.24.28
HPLC法同时测定云连药材中6种生物碱的含量
Simultaneous Determination of 6 Alkaloids in Coptis teeta by HPLC
摘要
Abstract
OBJECTIVE:To develop a method for simultaneous determination of 6 alkaloids in Coptis teeta.METHODS:The determination was performed on XtimateTM C18 with mobile phase consisted of 30 mmol/L ammonium bicarbonate [containing 0.1％ triethylamine and 0.7％ ammonia-acetonitrile (gradient elution)] at the flow rate of 1.0 mL/min.The detection wavelength was set at 270 nm,the column temperature was 30 ℃,and the sample size was 10 μL.RESULTS:The linear ranges of jateorrhizine,columbamine,epiberberine,coptisine,palmatine hydrochloride and berberine hydrochloride were 0.85-16.96 mg/L (r=0.999 9),1.25-24.96 mg/L(r=0.999 8),2.05-40.96 mg/L(r=0.999 9),3.65-72.96 mg/L(r=0.999 9),2.88-57.60 mg/L(r=0.999 9) and 13.25-264.96 mg/L(r=0.999 9),respectively.RSDs of precision,stability and reproducibility tests were all lower than 3.0％.Recoveries were 97.14％-102.14％ (RSD=1.93％,n=6),97.00％-102.00％ (RSD=2.06％,n=6),98.18％-101.82 ％ (RSD=1.79％,n=6),96.15％-101.28％ (RSD=2.06％,n=6),96.88％-101.88％ (RSD=1.87％,n=6),99.31％-103.76％ (RSD=1.89％,n=6),respectively.CONCLUSIONS:The method is simple,accurate,stability and reproducible,and can be used for simultaneous determination of 6 alkaloids in Coptis teeta.关键词
云连/生物碱/含量测定/高效液相色谱法Key words
Coptis teeta/Alkaloids/Content determination/HPLC分类
医药卫生引用本文复制引用
潘正,高运玲,江生,范刚,张艺,曹纬国..HPLC法同时测定云连药材中6种生物碱的含量[J].中国药房,2017,28(24):3408-3411,4.基金项目
重庆市基础与前沿研究计划项目(No.cstc2014jcyjA10004) （No.cstc2014jcyjA10004）
重庆市教委科学技术研究项目(No.KJ1400433) （No.KJ1400433）
重庆市卫生计生委中医药科技项目(No.ZY201602066) （No.ZY201602066）
