中国药房2017，Vol.28Issue(24)：3422-3425,4.DOI:10.6039/j.issn.1001-0408.2017.24.32
RP-HPLC法同时测定五酯片中10种木脂素类成分的含量
Simultaneous Determination of 10 Lignanoids in Wuzhi Tablets by RP-HPLC
摘要
Abstract
OBJECTIVE:To establish a method for content determination of schizantherin E,gomisin J,angeloylgomisin H,schisantherin A,schisantherin B,schisanhenol,anwuligan,schizandrin A,schizandrin B and schizandrin C in Wuzhi tablets.METHODS:RP-HPLC method was adopted.The determination was performed on Symmetry C18 column with acetonitrile-0.1％ phosphoric acid solution (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set 225 rm,and the column temperature was 30 ℃.The sample size was 10 μL.RESULTS:The linear ranges of schizantherin E,gomisin J,angeloylgomisin H,schisantherin A,schisantherin B,schisanhenol,anwuligan,schizandrin A,schizandrin B and schizandrin C were 2.25-67.5ng(r=0.999 6),2.1-63 ng(r=0.999 8),28-840 ng(r=0.999 9),124.6-3 738 ng(r=0.999 9),22.7-681 ng(r=0.999 9),32.7-981 ng(r=0.999 9),47-1 410 ng(r=0.999 9),208-6 240 ng(r=0.999 9),5.36-160.8 rig(r=0.999 9),4.48-134.4 ng(r=0.999 8).The limits of quantitation were 14.17,13.32,9.33,11.37,14.62,19.88,14.66,12.50,16.40,13.55 rg.The limits of detection were 4.62,4.60,3.08,3.76,4.81,6.74,4.93,4.16,5.86,5.03 ng.RSDs of precision,stability and reproducibility tests were less than 3.0％;the recoveries were 96.36％-100.00％(RSD=1.83％,n=6),95.00％-100.00％(RSD=2.07％,n=6),95.00％-98.00％(RSD=1.22％,n=6),95.37％-98.91％ (RSD=1.29％,n=6),95.62 ％-103.71％ (RSD=2.85％,n=6),97.33％-102.67％ (RSD=2.00％,n=6),95.00％-99.33％ (RSD=1.75％,n=6),97.24％-104.93％ (RSD=2.63％,n=6),95.00％-97.50％ (RSD=1.42％,n=6),96.00％-102.00％ (RSD=2.45％,n=6),respectively.CONCLUSIONS:The developed method is accurate,sensitive and reproducible,and it can be used for content determination of 10 lignanoids in Wuzhi tablets.关键词
五酯片/木脂素/反相高效液相色谱法/含量测定Key words
Wuzhi tablet/Lignanoids/RP-HPLC/Content determination分类
医药卫生引用本文复制引用
张琳,窦志华,蔡卫华,王建新,陈霞,王征宇..RP-HPLC法同时测定五酯片中10种木脂素类成分的含量[J].中国药房,2017,28(24):3422-3425,4.基金项目
江苏省药学会Shire生物药学基金立项课题(No.S201608) （No.S201608）
南通市卫生计生委青年医学人才科研立项课题(No.WQ2016017) （No.WQ2016017）
南通市科技计划(指导性)项目(No.YYZ16017) （指导性）
南通市科技计划项目(No.MS22015086) （No.MS22015086）
