中国中医药信息杂志2017，Vol.24Issue(10)：35-39,5.DOI:10.3969/j.issn.1005-5304.2017.10.009
左归降糖解郁方对模拟糖尿病并发抑郁症环境下模型大鼠海马神经元凋亡相关蛋白的影响
Effects of Zuogui Jiangtang Jieyu Formula on Hippocampus Neuron Apoptosis Related Proteins under Diabetes Mellitus with Depression States
摘要
Abstract
Objective To investigate the regulating effects of Zuogui Jiangtang Jieyu Formula (ZJJF) on Bcl-2, Bax and Caspase-8 in hippocampus neuron damage in diabetes mellitus with depression (DD). Methods Hippocampal neurons from 18 d pregnant rats were primitively cultivated, and then combination of glucose and corticosterone was used to construct DD simulation environment. Cultivated hippocampal neurons were randomly divided into normal group, blank serum group, model group, positive medicine (metformin+fluoxetine) serum group and to-be-tested medicine (ZJJF) serum group. Normal group and model group were given same amount culture medium, while other group were given relevant amont of 10％ medicine serum or blank serum. After modeling intervention for 18 h,Hoechst staining was used to detect the apoptosis of hippocampal neurons. The expression of Bcl-2, Bax and Caspase-8 was detected by high content analysis. Results Compared with the control group, hippocampal neuron dendrites ruptured or decreased, neural network connection decreased, cells showed significant staining, broken, uneven distribution of light spots, the expression of Bcl-2 protein decreased significantly (P<0.05), but Bax and Caspase-8 were increased (P<0.05, P<0.01). Compared with the model group, hippocampal neurons in both positive medicine serum group and ZJJF serum group gradually recovered. Hoechst staining showed that the nuclei were significantly homogenized, local highlights were significantly reduced, Bcl-2 protein expression levels were significantly increased (P<0.05, P<0.01), while Bax and Caspase-8 were obviously down-regulated (P<0.05, P<0.01). Conclusion ZJJF has protective effects on hippocampal neurons in DD of model rats, and its mechanism is related to regulating the expression of Bcl-2, Bax and Caspase-8 in hippocampus neuron.关键词
左归降糖解郁方/糖尿病并发抑郁症/海马神经元/Bcl-2/Bax/Caspase-8/大鼠Key words
Zuogui Jiangtang Jieyu Formula/diabetes mellitus with depression/hippocampal neurons/Bcl-2/Bax/Caspase-8/rats分类
医药卫生引用本文复制引用
凌佳,杨琴,刘检,王宇红,徐雅岚,韩远山,杨蕙,孟盼..左归降糖解郁方对模拟糖尿病并发抑郁症环境下模型大鼠海马神经元凋亡相关蛋白的影响[J].中国中医药信息杂志,2017,24(10):35-39,5.基金项目
国家自然科学基金(81373578、81573965、81403379、81403387) （81373578、81573965、81403379、81403387）
