中山大学学报（医学科学版）2017，Vol.38Issue(5)：665-669,5.
羟基红花黄色素A对脓毒症小鼠外周血促炎/抗炎因子的影响
Effect of Hydroxysafflor Yellow A on Pro/Anti-Inflammatory Cytokines in Peripheral Blood with Sepsis in Mice
摘要
Abstract
[Objective]To explore the effect of HSYA on pro/anti-inflammatory cytokine′s levels and mRNA expression in peripheral blood of mice.with sepsis.[Methods]Dividing NIH mice into four groups,as normal group,sham group,CLP group and HSYA group,24 mice in each group. The CLP sepsis mouse model was established. HSYA(120 mg/kg)were injected intravenously at 12 h before the operation ,and 0 h and 12 h following CLP ,and other groups were given normal saline. observed the animals behavior changes,measured the levels WBC,PLT,ALT,AST,BUN in serum,detected the levels and mRNA expression of IL-6, IL-10 ,TNF-α in peripheral blood. cultured of peripheral blood bacteria loads.[Results]24 h after surgery ,mice in CLP group appeared furring,feces residues on anus etc. compared to normal group,sham group and HSYA group,WBC,PLT,ALT,AST, BUN,levels and mRNA expression of IL-6,IL-10,TNF-αshowed significant increases,it was also found that bacterial load was significant increased in model group.[Conclusions]HSYA has a therapeutic effect in mice with sepsis ,can reduce bacteria into the blood,and inhibit inflammatory mediators which caused tissue damage.关键词
脓毒症/羟基红花黄色素A/炎症介质/细菌负荷Key words
sepsis/hydroxysafflor yellow a/inflammatory cytokine/bacterial load分类
医药卫生引用本文复制引用
王金平,王平,陈润桦,赵佩研,黄泽愉,吴汉伟,吴建龙..羟基红花黄色素A对脓毒症小鼠外周血促炎/抗炎因子的影响[J].中山大学学报（医学科学版）,2017,38(5):665-669,5.基金项目
国家自然科学基金(81503320) （81503320）
深圳市科创委基础研究(JCYJ20150330102401101) （JCYJ20150330102401101）
