中山大学学报（医学科学版）2017，Vol.38Issue(5)：670-675,6.
旋毛虫排泄分泌抗原53-ku重组蛋白上调M2型肺泡巨噬细胞减轻脓毒症小鼠急性肺损伤
Recombinant Trichinella Spiralis Excretory-secretory Antigen 53ku Protein Alleviates Acute Lung Injury in Septic Mice via Polarization of M2 Macrophages
摘要
Abstract
[Objectives]To investigate the protective effects of recombinant Trichinella spiralis excretory-secretory 53ku pro-tein(rTsP53)on acute lung injuries in mice.[Methods]Thirty Balb/c mice were randomly divided into normal group. ALI group and rTsP53 group(n=10,respectively). Macrophages were harvested by bronchoalveolar lavage. Mortality in 72 hours was counted and compared. Pathological damage of lung tissues was observed by HE staining and graded by Smith score. Wet/dry ratio was measured. The bronchoalveolar lavage fluid concertration of IL-6 and IL-4 was measured by ELISA. The mRNA expression of TNF-α,iNOS, IL-10 and Arg-1 in alveolar lavage macrophages was detected by RT-PCR.[Results]72 h mortality of ALI mice was 70%,which was reduced to 30% in mice received rTsP53 treatment. Compared with ALI mice,the pathological damage of in rTsP53 treated-mice was improved and Smith score was declined ,combined with descending W/D ratio. IL-6 level of alveolar lavage fluid was elevated in ALI mice compared with normal group. And alveolar lavage macrophage was polarized to M2 sub-type,appeared as higher mRNA expression of TNF-α and iNOS and lower level of IL-10 and Arg-1. Bronchoalveolar lavage fluid concentration of IL-6 was declined and IL-4 was elevated in rTsP53-treated mice compared with ALI group. The macrophages of alveolar wash had higher mRNA expression of IL-10 and Arg-1,while lower level of TNF-α and iNOS,manifesting M2 polarization characteristics.[Conclusion]Recombinant T.spiralis P53 protein could protect mice from acute lung injuries induced by LPS via modulating M2 macrophage polarization,which play a role in depression of inflammatory reaction and tissue repairment.关键词
重组旋毛虫排泄分泌抗原53-ku蛋白/脂多糖/急性肺损伤/M2型巨噬细胞Key words
rTsP53/lipopolysaccharide/acute lung injury/M2 macrophages分类
医药卫生引用本文复制引用
关开泮,江仁,徐雪,刘志豪,廖瑾莉,熊艳,詹红,曾庆理,徐嘉..旋毛虫排泄分泌抗原53-ku重组蛋白上调M2型肺泡巨噬细胞减轻脓毒症小鼠急性肺损伤[J].中山大学学报（医学科学版）,2017,38(5):670-675,6.基金项目
广东省医学科学技术研究基金(A2015486,B2014110) （A2015486,B2014110）
广东省自然科学基金(2016A030313249) （2016A030313249）
广东省科技计划项目(2014A020212150) （2014A020212150）
