中山大学学报（医学科学版）2017，Vol.38Issue(5)：676-684,9.
孟鲁司特抑制呼吸道合胞病毒感染人支气管上皮细胞所致炎症的药理机制
Montelukast Inhibits the Inflammation of Human Bronchial Epithelial Cells Infected by Respiratory Syncytial Virus
摘要
Abstract
[Objective]To investigate the pharmacological mechanism of montelukast in the inhibition of inflammation.[Meth-ods]Respiratory syncytial virus-infected human bronchial epithelial cell(16HBEC)inflammatory cell model was established,and mRNA and protein expressions of Nuclear factor NF-E2 related factor(Nrf2),heme oxygenase(HO-1),quinone oxidoreductase (NQO-1),and glutathione transferase (GST) were determined by qPCR and Western-blot ,and production of cellular reactive oxygen species(ROS)was measured by DCFH-DA fluorescent probe method. Nrf2 siRNA was further synthesized to reduce the expression of Nrf2 ,to investigate the chang of inflammatory index.[Results]Montelukast significantly reduced the expressions of inflammatory cytokines IL-6,TNF-α,and IL-1β(P<0.05)on respiratory syncytial virus-infected 16HBEC,and the ROS level in inflammatory cell model was decreased(P<0.05),increased the mRNA and protein expressions of Nrf2,HO-1,NQO-1 and GST (P < 0.05),with a more significant effect at higher dose. After the down-regulation of Nrf2,the expressions of inflammatory cyto-kines IL-6,TNF-α,and IL-1βwere increased(P<0.05),and ROS level was significantly increased(P<0.05),mRNA and pro-tein expressions of Nrf2,HO-1,NQO-1 and GST were decreased(P<0.05).[Conclusion]Montelukast inhibits the inflammation of human bronchial epithelial cells infected by respiratory syncytial virus (RSV),and the potential mechanism may involve its ef-fect on the Nrf2/ARE signaling pathway.关键词
孟鲁司特/呼吸道合胞病毒感染/炎症/Nrf2/ARE信号通路Key words
montelukast/respiratory syncytial virus infection/inflammation/Nrf2/ARE signaling pathway分类
医药卫生引用本文复制引用
江敏时,檀卫平,陈小平,姚海魂..孟鲁司特抑制呼吸道合胞病毒感染人支气管上皮细胞所致炎症的药理机制[J].中山大学学报（医学科学版）,2017,38(5):676-684,9.基金项目
广东省医学科研基金(B2013120) （B2013120）
